Review



rabbit anti-par-3  (Millipore)


Bioz Verified Symbol Millipore is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 90

    Structured Review

    Millipore rabbit anti-par-3
    Rabbit Anti Par 3, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti-par-3/par3+antibody/pmc12077515-214-31-32
    Average 90 stars, based on 1 article reviews
    rabbit anti-par-3 - by Bioz Stars, 2026-10
    90/100 stars

    Images

    Related Articles

    Immunofluorescence:


    Article Title: Polarized endosome dynamics engage cytosolic Par-3 and dynein during asymmetric division
    Article Snippet: Primary antibodies used in this study were: mouse anti-Dld (Abcam ab73331; RRID:AB_1268496; lot GR115501-3, 1:200 dilution for immunostaining) , chicken anti-GFP (Abcam ab13970; RRID:AB_300798, lot GR3190550-20, 1:500 dilution for immunostaining), rabbit anti-Par-3 (Millipore 07-330; RRID:AB_2101325; lot 3322358, 1:500 for immunostaining)(validated in this study), guinea pig anti-DLIC-Cter (a gift from D. T. Uemura, 1:100 for immunostaining and 1:500 for western blotting, validated in this study) , rabbit anti-α-Tubulin (Santa Cruz SC-12462; RRID:AB_2241125, lot A2907, 1:1000 for western blotting).

    Article Title: Nodal Dependent Differential Localisation of Dishevelled-2 Demarcates Regions of Differing Cell Behaviour in the Visceral Endoderm
    Article Snippet: Primary antibodies used were: Rabbit anti-ZO-1 (Zymed laboratories 61-7300) 1∶100, Rabbit anti-Myosin IIA (Sigma M8064) 1∶100, Rat Anti-Uvomorulin/E-cadherin (Sigma U3254) 1∶200, Rabbit anti-Dishevelled-2 (Biomol 4270) 1∶200, Sheep anti-Vangl2 (R&D Systems AF4815) 1∶100, and Rabbit anti-Par-3 (Millipore 07-330) 1∶100.

    Article Title: EPH Receptor Tyrosine Kinases Regulate Epithelial Morphogenesis and Phosphorylate the PAR-3 Scaffold Protein to Modulate Downstream Signaling Networks
    Article Snippet: Primary antibodies used were: rabbit anti-FLAG (Sigma-Aldrich, #F7425), mouse anti-pan phosphotyrosine 4G10 (Millipore, #05-321), mouse anti-GFP (Abcam, #290), mouse anti-EPHA4 (BD Biosciences, #610471), goat anti-EPHB2 (R&D Systems, # AF467), goat anti-EPHA1 (R&D Systems, #AF638), mouse anti-EPHA2 (Millipore, #05-480), goat anti-EPHB4 (R&D Systems, #AF3038), mouse anti-PKCι (BD, #610175), rabbit anti-CSK (Cell Signaling Technology, #4980), rabbit anti-PAR-3 (Millipore, #07-330), mouse anti-actin (Cell Signaling Technology, #3700).

    Article Title: Phosphorylation by Aurora kinase A facilitates cortical-cytoplasmic dynamics of Par-3 in asymmetric division of radial glia progenitors
    Article Snippet: Zebrafish embryos were treated with an AurkA inhibitor (MedChemExpress, MK-5108, HY-13252, CAS no. 1010085-13-8) from 22 to 24 hpf for various durations before imaging (10, 20, 30, 40, 50, 60, and 120 min).

    Article Title: EPH receptor tyrosine kinases phosphorylate the PAR-3 scaffold protein to modulate downstream signaling networks.
    Article Snippet: Primary antibodies used were: rabbit anti-FLAG (Sigma-Aldrich, #F7425), mouse anti-pan phosphotyrosine 4G10 (Sigma-Aldrich, #05–321), mouse anti-GFP (Thermo Fisher Scientific, #A-11122, Roche, #1181446001), mouse anti-EPHA4 (BD Biosciences, #610471), goat anti-EPHB2 (R&D Systems, # AF467), rabbit anti-CSK (Cell Signaling Technology, #4980), rabbit anti-PAR-3 (Millipore, #07–330), rabbit anti-LIMK2 (Cell Signaling Technology, #3845), rabbit anti-phospho-SRC (Tyr527) (Cell Signaling Technology, #2105), mouse anti-SRC (Cell Signaling Technology, #2110) mouse anti-Actin (Cell Signaling Technology, #3700), mouse anti-Tubulin (Cell Signaling Technology, #3873).

    Staining:


    Article Title: Polarized endosome dynamics engage cytosolic Par-3 and dynein during asymmetric division
    Article Snippet: Primary antibodies used in this study were: mouse anti-Dld (Abcam ab73331; RRID:AB_1268496; lot GR115501-3, 1:200 dilution for immunostaining) , chicken anti-GFP (Abcam ab13970; RRID:AB_300798, lot GR3190550-20, 1:500 dilution for immunostaining), rabbit anti-Par-3 (Millipore 07-330; RRID:AB_2101325; lot 3322358, 1:500 for immunostaining)(validated in this study), guinea pig anti-DLIC-Cter (a gift from D. T. Uemura, 1:100 for immunostaining and 1:500 for western blotting, validated in this study) , rabbit anti-α-Tubulin (Santa Cruz SC-12462; RRID:AB_2241125, lot A2907, 1:1000 for western blotting).

    Article Title: Nodal Dependent Differential Localisation of Dishevelled-2 Demarcates Regions of Differing Cell Behaviour in the Visceral Endoderm
    Article Snippet: Primary antibodies used were: Rabbit anti-ZO-1 (Zymed laboratories 61-7300) 1∶100, Rabbit anti-Myosin IIA (Sigma M8064) 1∶100, Rat Anti-Uvomorulin/E-cadherin (Sigma U3254) 1∶200, Rabbit anti-Dishevelled-2 (Biomol 4270) 1∶200, Sheep anti-Vangl2 (R&D Systems AF4815) 1∶100, and Rabbit anti-Par-3 (Millipore 07-330) 1∶100.

    Article Title: EPH Receptor Tyrosine Kinases Regulate Epithelial Morphogenesis and Phosphorylate the PAR-3 Scaffold Protein to Modulate Downstream Signaling Networks
    Article Snippet: Primary antibodies used were: rabbit anti-FLAG (Sigma-Aldrich, #F7425), mouse anti-pan phosphotyrosine 4G10 (Millipore, #05-321), mouse anti-GFP (Abcam, #290), mouse anti-EPHA4 (BD Biosciences, #610471), goat anti-EPHB2 (R&D Systems, # AF467), goat anti-EPHA1 (R&D Systems, #AF638), mouse anti-EPHA2 (Millipore, #05-480), goat anti-EPHB4 (R&D Systems, #AF3038), mouse anti-PKCι (BD, #610175), rabbit anti-CSK (Cell Signaling Technology, #4980), rabbit anti-PAR-3 (Millipore, #07-330), mouse anti-actin (Cell Signaling Technology, #3700).

    Article Title: Phosphorylation by Aurora kinase A facilitates cortical-cytoplasmic dynamics of Par-3 in asymmetric division of radial glia progenitors
    Article Snippet: Zebrafish embryos were treated with an AurkA inhibitor (MedChemExpress, MK-5108, HY-13252, CAS no. 1010085-13-8) from 22 to 24 hpf for various durations before imaging (10, 20, 30, 40, 50, 60, and 120 min).

    Article Title: EPH receptor tyrosine kinases phosphorylate the PAR-3 scaffold protein to modulate downstream signaling networks.
    Article Snippet: Primary antibodies used were: rabbit anti-FLAG (Sigma-Aldrich, #F7425), mouse anti-pan phosphotyrosine 4G10 (Sigma-Aldrich, #05–321), mouse anti-GFP (Thermo Fisher Scientific, #A-11122, Roche, #1181446001), mouse anti-EPHA4 (BD Biosciences, #610471), goat anti-EPHB2 (R&D Systems, # AF467), rabbit anti-CSK (Cell Signaling Technology, #4980), rabbit anti-PAR-3 (Millipore, #07–330), rabbit anti-LIMK2 (Cell Signaling Technology, #3845), rabbit anti-phospho-SRC (Tyr527) (Cell Signaling Technology, #2105), mouse anti-SRC (Cell Signaling Technology, #2110) mouse anti-Actin (Cell Signaling Technology, #3700), mouse anti-Tubulin (Cell Signaling Technology, #3873).

    Co-Culture Assay:


    Article Title: Polarized endosome dynamics engage cytosolic Par-3 and dynein during asymmetric division
    Article Snippet: Primary antibodies used in this study were: mouse anti-Dld (Abcam ab73331; RRID:AB_1268496; lot GR115501-3, 1:200 dilution for immunostaining) , chicken anti-GFP (Abcam ab13970; RRID:AB_300798, lot GR3190550-20, 1:500 dilution for immunostaining), rabbit anti-Par-3 (Millipore 07-330; RRID:AB_2101325; lot 3322358, 1:500 for immunostaining)(validated in this study), guinea pig anti-DLIC-Cter (a gift from D. T. Uemura, 1:100 for immunostaining and 1:500 for western blotting, validated in this study) , rabbit anti-α-Tubulin (Santa Cruz SC-12462; RRID:AB_2241125, lot A2907, 1:1000 for western blotting).

    Article Title: Nodal Dependent Differential Localisation of Dishevelled-2 Demarcates Regions of Differing Cell Behaviour in the Visceral Endoderm
    Article Snippet: Primary antibodies used were: Rabbit anti-ZO-1 (Zymed laboratories 61-7300) 1∶100, Rabbit anti-Myosin IIA (Sigma M8064) 1∶100, Rat Anti-Uvomorulin/E-cadherin (Sigma U3254) 1∶200, Rabbit anti-Dishevelled-2 (Biomol 4270) 1∶200, Sheep anti-Vangl2 (R&D Systems AF4815) 1∶100, and Rabbit anti-Par-3 (Millipore 07-330) 1∶100.

    Article Title: EPH Receptor Tyrosine Kinases Regulate Epithelial Morphogenesis and Phosphorylate the PAR-3 Scaffold Protein to Modulate Downstream Signaling Networks
    Article Snippet: Primary antibodies used were: rabbit anti-FLAG (Sigma-Aldrich, #F7425), mouse anti-pan phosphotyrosine 4G10 (Millipore, #05-321), mouse anti-GFP (Abcam, #290), mouse anti-EPHA4 (BD Biosciences, #610471), goat anti-EPHB2 (R&D Systems, # AF467), goat anti-EPHA1 (R&D Systems, #AF638), mouse anti-EPHA2 (Millipore, #05-480), goat anti-EPHB4 (R&D Systems, #AF3038), mouse anti-PKCι (BD, #610175), rabbit anti-CSK (Cell Signaling Technology, #4980), rabbit anti-PAR-3 (Millipore, #07-330), mouse anti-actin (Cell Signaling Technology, #3700).

    Article Title: Phosphorylation by Aurora kinase A facilitates cortical-cytoplasmic dynamics of Par-3 in asymmetric division of radial glia progenitors
    Article Snippet: Zebrafish embryos were treated with an AurkA inhibitor (MedChemExpress, MK-5108, HY-13252, CAS no. 1010085-13-8) from 22 to 24 hpf for various durations before imaging (10, 20, 30, 40, 50, 60, and 120 min).

    Article Title: EPH receptor tyrosine kinases phosphorylate the PAR-3 scaffold protein to modulate downstream signaling networks.
    Article Snippet: Primary antibodies used were: rabbit anti-FLAG (Sigma-Aldrich, #F7425), mouse anti-pan phosphotyrosine 4G10 (Sigma-Aldrich, #05–321), mouse anti-GFP (Thermo Fisher Scientific, #A-11122, Roche, #1181446001), mouse anti-EPHA4 (BD Biosciences, #610471), goat anti-EPHB2 (R&D Systems, # AF467), rabbit anti-CSK (Cell Signaling Technology, #4980), rabbit anti-PAR-3 (Millipore, #07–330), rabbit anti-LIMK2 (Cell Signaling Technology, #3845), rabbit anti-phospho-SRC (Tyr527) (Cell Signaling Technology, #2105), mouse anti-SRC (Cell Signaling Technology, #2110) mouse anti-Actin (Cell Signaling Technology, #3700), mouse anti-Tubulin (Cell Signaling Technology, #3873).

    Functional Assay:


    Article Title: Polarized endosome dynamics engage cytosolic Par-3 and dynein during asymmetric division
    Article Snippet: Primary antibodies used in this study were: mouse anti-Dld (Abcam ab73331; RRID:AB_1268496; lot GR115501-3, 1:200 dilution for immunostaining) , chicken anti-GFP (Abcam ab13970; RRID:AB_300798, lot GR3190550-20, 1:500 dilution for immunostaining), rabbit anti-Par-3 (Millipore 07-330; RRID:AB_2101325; lot 3322358, 1:500 for immunostaining)(validated in this study), guinea pig anti-DLIC-Cter (a gift from D. T. Uemura, 1:100 for immunostaining and 1:500 for western blotting, validated in this study) , rabbit anti-α-Tubulin (Santa Cruz SC-12462; RRID:AB_2241125, lot A2907, 1:1000 for western blotting).

    Article Title: Nodal Dependent Differential Localisation of Dishevelled-2 Demarcates Regions of Differing Cell Behaviour in the Visceral Endoderm
    Article Snippet: Primary antibodies used were: Rabbit anti-ZO-1 (Zymed laboratories 61-7300) 1∶100, Rabbit anti-Myosin IIA (Sigma M8064) 1∶100, Rat Anti-Uvomorulin/E-cadherin (Sigma U3254) 1∶200, Rabbit anti-Dishevelled-2 (Biomol 4270) 1∶200, Sheep anti-Vangl2 (R&D Systems AF4815) 1∶100, and Rabbit anti-Par-3 (Millipore 07-330) 1∶100.

    Article Title: EPH Receptor Tyrosine Kinases Regulate Epithelial Morphogenesis and Phosphorylate the PAR-3 Scaffold Protein to Modulate Downstream Signaling Networks
    Article Snippet: Primary antibodies used were: rabbit anti-FLAG (Sigma-Aldrich, #F7425), mouse anti-pan phosphotyrosine 4G10 (Millipore, #05-321), mouse anti-GFP (Abcam, #290), mouse anti-EPHA4 (BD Biosciences, #610471), goat anti-EPHB2 (R&D Systems, # AF467), goat anti-EPHA1 (R&D Systems, #AF638), mouse anti-EPHA2 (Millipore, #05-480), goat anti-EPHB4 (R&D Systems, #AF3038), mouse anti-PKCι (BD, #610175), rabbit anti-CSK (Cell Signaling Technology, #4980), rabbit anti-PAR-3 (Millipore, #07-330), mouse anti-actin (Cell Signaling Technology, #3700).

    Article Title: Phosphorylation by Aurora kinase A facilitates cortical-cytoplasmic dynamics of Par-3 in asymmetric division of radial glia progenitors
    Article Snippet: Zebrafish embryos were treated with an AurkA inhibitor (MedChemExpress, MK-5108, HY-13252, CAS no. 1010085-13-8) from 22 to 24 hpf for various durations before imaging (10, 20, 30, 40, 50, 60, and 120 min).

    Article Title: EPH receptor tyrosine kinases phosphorylate the PAR-3 scaffold protein to modulate downstream signaling networks.
    Article Snippet: Primary antibodies used were: rabbit anti-FLAG (Sigma-Aldrich, #F7425), mouse anti-pan phosphotyrosine 4G10 (Sigma-Aldrich, #05–321), mouse anti-GFP (Thermo Fisher Scientific, #A-11122, Roche, #1181446001), mouse anti-EPHA4 (BD Biosciences, #610471), goat anti-EPHB2 (R&D Systems, # AF467), rabbit anti-CSK (Cell Signaling Technology, #4980), rabbit anti-PAR-3 (Millipore, #07–330), rabbit anti-LIMK2 (Cell Signaling Technology, #3845), rabbit anti-phospho-SRC (Tyr527) (Cell Signaling Technology, #2105), mouse anti-SRC (Cell Signaling Technology, #2110) mouse anti-Actin (Cell Signaling Technology, #3700), mouse anti-Tubulin (Cell Signaling Technology, #3873).

    Expressing:


    Article Title: Polarized endosome dynamics engage cytosolic Par-3 and dynein during asymmetric division
    Article Snippet: Primary antibodies used in this study were: mouse anti-Dld (Abcam ab73331; RRID:AB_1268496; lot GR115501-3, 1:200 dilution for immunostaining) , chicken anti-GFP (Abcam ab13970; RRID:AB_300798, lot GR3190550-20, 1:500 dilution for immunostaining), rabbit anti-Par-3 (Millipore 07-330; RRID:AB_2101325; lot 3322358, 1:500 for immunostaining)(validated in this study), guinea pig anti-DLIC-Cter (a gift from D. T. Uemura, 1:100 for immunostaining and 1:500 for western blotting, validated in this study) , rabbit anti-α-Tubulin (Santa Cruz SC-12462; RRID:AB_2241125, lot A2907, 1:1000 for western blotting).

    Article Title: Nodal Dependent Differential Localisation of Dishevelled-2 Demarcates Regions of Differing Cell Behaviour in the Visceral Endoderm
    Article Snippet: Primary antibodies used were: Rabbit anti-ZO-1 (Zymed laboratories 61-7300) 1∶100, Rabbit anti-Myosin IIA (Sigma M8064) 1∶100, Rat Anti-Uvomorulin/E-cadherin (Sigma U3254) 1∶200, Rabbit anti-Dishevelled-2 (Biomol 4270) 1∶200, Sheep anti-Vangl2 (R&D Systems AF4815) 1∶100, and Rabbit anti-Par-3 (Millipore 07-330) 1∶100.

    Article Title: EPH Receptor Tyrosine Kinases Regulate Epithelial Morphogenesis and Phosphorylate the PAR-3 Scaffold Protein to Modulate Downstream Signaling Networks
    Article Snippet: Primary antibodies used were: rabbit anti-FLAG (Sigma-Aldrich, #F7425), mouse anti-pan phosphotyrosine 4G10 (Millipore, #05-321), mouse anti-GFP (Abcam, #290), mouse anti-EPHA4 (BD Biosciences, #610471), goat anti-EPHB2 (R&D Systems, # AF467), goat anti-EPHA1 (R&D Systems, #AF638), mouse anti-EPHA2 (Millipore, #05-480), goat anti-EPHB4 (R&D Systems, #AF3038), mouse anti-PKCι (BD, #610175), rabbit anti-CSK (Cell Signaling Technology, #4980), rabbit anti-PAR-3 (Millipore, #07-330), mouse anti-actin (Cell Signaling Technology, #3700).

    Article Title: Phosphorylation by Aurora kinase A facilitates cortical-cytoplasmic dynamics of Par-3 in asymmetric division of radial glia progenitors
    Article Snippet: Zebrafish embryos were treated with an AurkA inhibitor (MedChemExpress, MK-5108, HY-13252, CAS no. 1010085-13-8) from 22 to 24 hpf for various durations before imaging (10, 20, 30, 40, 50, 60, and 120 min).

    Article Title: EPH receptor tyrosine kinases phosphorylate the PAR-3 scaffold protein to modulate downstream signaling networks.
    Article Snippet: Primary antibodies used were: rabbit anti-FLAG (Sigma-Aldrich, #F7425), mouse anti-pan phosphotyrosine 4G10 (Sigma-Aldrich, #05–321), mouse anti-GFP (Thermo Fisher Scientific, #A-11122, Roche, #1181446001), mouse anti-EPHA4 (BD Biosciences, #610471), goat anti-EPHB2 (R&D Systems, # AF467), rabbit anti-CSK (Cell Signaling Technology, #4980), rabbit anti-PAR-3 (Millipore, #07–330), rabbit anti-LIMK2 (Cell Signaling Technology, #3845), rabbit anti-phospho-SRC (Tyr527) (Cell Signaling Technology, #2105), mouse anti-SRC (Cell Signaling Technology, #2110) mouse anti-Actin (Cell Signaling Technology, #3700), mouse anti-Tubulin (Cell Signaling Technology, #3873).

    shRNA:


    Article Title: Polarized endosome dynamics engage cytosolic Par-3 and dynein during asymmetric division
    Article Snippet: Primary antibodies used in this study were: mouse anti-Dld (Abcam ab73331; RRID:AB_1268496; lot GR115501-3, 1:200 dilution for immunostaining) , chicken anti-GFP (Abcam ab13970; RRID:AB_300798, lot GR3190550-20, 1:500 dilution for immunostaining), rabbit anti-Par-3 (Millipore 07-330; RRID:AB_2101325; lot 3322358, 1:500 for immunostaining)(validated in this study), guinea pig anti-DLIC-Cter (a gift from D. T. Uemura, 1:100 for immunostaining and 1:500 for western blotting, validated in this study) , rabbit anti-α-Tubulin (Santa Cruz SC-12462; RRID:AB_2241125, lot A2907, 1:1000 for western blotting).

    Article Title: Nodal Dependent Differential Localisation of Dishevelled-2 Demarcates Regions of Differing Cell Behaviour in the Visceral Endoderm
    Article Snippet: Primary antibodies used were: Rabbit anti-ZO-1 (Zymed laboratories 61-7300) 1∶100, Rabbit anti-Myosin IIA (Sigma M8064) 1∶100, Rat Anti-Uvomorulin/E-cadherin (Sigma U3254) 1∶200, Rabbit anti-Dishevelled-2 (Biomol 4270) 1∶200, Sheep anti-Vangl2 (R&D Systems AF4815) 1∶100, and Rabbit anti-Par-3 (Millipore 07-330) 1∶100.

    Article Title: EPH Receptor Tyrosine Kinases Regulate Epithelial Morphogenesis and Phosphorylate the PAR-3 Scaffold Protein to Modulate Downstream Signaling Networks
    Article Snippet: Primary antibodies used were: rabbit anti-FLAG (Sigma-Aldrich, #F7425), mouse anti-pan phosphotyrosine 4G10 (Millipore, #05-321), mouse anti-GFP (Abcam, #290), mouse anti-EPHA4 (BD Biosciences, #610471), goat anti-EPHB2 (R&D Systems, # AF467), goat anti-EPHA1 (R&D Systems, #AF638), mouse anti-EPHA2 (Millipore, #05-480), goat anti-EPHB4 (R&D Systems, #AF3038), mouse anti-PKCι (BD, #610175), rabbit anti-CSK (Cell Signaling Technology, #4980), rabbit anti-PAR-3 (Millipore, #07-330), mouse anti-actin (Cell Signaling Technology, #3700).

    Article Title: Phosphorylation by Aurora kinase A facilitates cortical-cytoplasmic dynamics of Par-3 in asymmetric division of radial glia progenitors
    Article Snippet: Zebrafish embryos were treated with an AurkA inhibitor (MedChemExpress, MK-5108, HY-13252, CAS no. 1010085-13-8) from 22 to 24 hpf for various durations before imaging (10, 20, 30, 40, 50, 60, and 120 min).

    Article Title: EPH receptor tyrosine kinases phosphorylate the PAR-3 scaffold protein to modulate downstream signaling networks.
    Article Snippet: Primary antibodies used were: rabbit anti-FLAG (Sigma-Aldrich, #F7425), mouse anti-pan phosphotyrosine 4G10 (Sigma-Aldrich, #05–321), mouse anti-GFP (Thermo Fisher Scientific, #A-11122, Roche, #1181446001), mouse anti-EPHA4 (BD Biosciences, #610471), goat anti-EPHB2 (R&D Systems, # AF467), rabbit anti-CSK (Cell Signaling Technology, #4980), rabbit anti-PAR-3 (Millipore, #07–330), rabbit anti-LIMK2 (Cell Signaling Technology, #3845), rabbit anti-phospho-SRC (Tyr527) (Cell Signaling Technology, #2105), mouse anti-SRC (Cell Signaling Technology, #2110) mouse anti-Actin (Cell Signaling Technology, #3700), mouse anti-Tubulin (Cell Signaling Technology, #3873).

    Western Blot:


    Article Title: Polarized endosome dynamics engage cytosolic Par-3 and dynein during asymmetric division
    Article Snippet: Primary antibodies used in this study were: mouse anti-Dld (Abcam ab73331; RRID:AB_1268496; lot GR115501-3, 1:200 dilution for immunostaining) , chicken anti-GFP (Abcam ab13970; RRID:AB_300798, lot GR3190550-20, 1:500 dilution for immunostaining), rabbit anti-Par-3 (Millipore 07-330; RRID:AB_2101325; lot 3322358, 1:500 for immunostaining)(validated in this study), guinea pig anti-DLIC-Cter (a gift from D. T. Uemura, 1:100 for immunostaining and 1:500 for western blotting, validated in this study) , rabbit anti-α-Tubulin (Santa Cruz SC-12462; RRID:AB_2241125, lot A2907, 1:1000 for western blotting).

    Article Title: Nodal Dependent Differential Localisation of Dishevelled-2 Demarcates Regions of Differing Cell Behaviour in the Visceral Endoderm
    Article Snippet: Primary antibodies used were: Rabbit anti-ZO-1 (Zymed laboratories 61-7300) 1∶100, Rabbit anti-Myosin IIA (Sigma M8064) 1∶100, Rat Anti-Uvomorulin/E-cadherin (Sigma U3254) 1∶200, Rabbit anti-Dishevelled-2 (Biomol 4270) 1∶200, Sheep anti-Vangl2 (R&D Systems AF4815) 1∶100, and Rabbit anti-Par-3 (Millipore 07-330) 1∶100.

    Article Title: EPH Receptor Tyrosine Kinases Regulate Epithelial Morphogenesis and Phosphorylate the PAR-3 Scaffold Protein to Modulate Downstream Signaling Networks
    Article Snippet: Primary antibodies used were: rabbit anti-FLAG (Sigma-Aldrich, #F7425), mouse anti-pan phosphotyrosine 4G10 (Millipore, #05-321), mouse anti-GFP (Abcam, #290), mouse anti-EPHA4 (BD Biosciences, #610471), goat anti-EPHB2 (R&D Systems, # AF467), goat anti-EPHA1 (R&D Systems, #AF638), mouse anti-EPHA2 (Millipore, #05-480), goat anti-EPHB4 (R&D Systems, #AF3038), mouse anti-PKCι (BD, #610175), rabbit anti-CSK (Cell Signaling Technology, #4980), rabbit anti-PAR-3 (Millipore, #07-330), mouse anti-actin (Cell Signaling Technology, #3700).

    Article Title: Phosphorylation by Aurora kinase A facilitates cortical-cytoplasmic dynamics of Par-3 in asymmetric division of radial glia progenitors
    Article Snippet: Zebrafish embryos were treated with an AurkA inhibitor (MedChemExpress, MK-5108, HY-13252, CAS no. 1010085-13-8) from 22 to 24 hpf for various durations before imaging (10, 20, 30, 40, 50, 60, and 120 min).

    Article Title: EPH receptor tyrosine kinases phosphorylate the PAR-3 scaffold protein to modulate downstream signaling networks.
    Article Snippet: Primary antibodies used were: rabbit anti-FLAG (Sigma-Aldrich, #F7425), mouse anti-pan phosphotyrosine 4G10 (Sigma-Aldrich, #05–321), mouse anti-GFP (Thermo Fisher Scientific, #A-11122, Roche, #1181446001), mouse anti-EPHA4 (BD Biosciences, #610471), goat anti-EPHB2 (R&D Systems, # AF467), rabbit anti-CSK (Cell Signaling Technology, #4980), rabbit anti-PAR-3 (Millipore, #07–330), rabbit anti-LIMK2 (Cell Signaling Technology, #3845), rabbit anti-phospho-SRC (Tyr527) (Cell Signaling Technology, #2105), mouse anti-SRC (Cell Signaling Technology, #2110) mouse anti-Actin (Cell Signaling Technology, #3700), mouse anti-Tubulin (Cell Signaling Technology, #3873).

    Cotransfection:


    Article Title: Polarized endosome dynamics engage cytosolic Par-3 and dynein during asymmetric division
    Article Snippet: Primary antibodies used in this study were: mouse anti-Dld (Abcam ab73331; RRID:AB_1268496; lot GR115501-3, 1:200 dilution for immunostaining) , chicken anti-GFP (Abcam ab13970; RRID:AB_300798, lot GR3190550-20, 1:500 dilution for immunostaining), rabbit anti-Par-3 (Millipore 07-330; RRID:AB_2101325; lot 3322358, 1:500 for immunostaining)(validated in this study), guinea pig anti-DLIC-Cter (a gift from D. T. Uemura, 1:100 for immunostaining and 1:500 for western blotting, validated in this study) , rabbit anti-α-Tubulin (Santa Cruz SC-12462; RRID:AB_2241125, lot A2907, 1:1000 for western blotting).

    Article Title: Nodal Dependent Differential Localisation of Dishevelled-2 Demarcates Regions of Differing Cell Behaviour in the Visceral Endoderm
    Article Snippet: Primary antibodies used were: Rabbit anti-ZO-1 (Zymed laboratories 61-7300) 1∶100, Rabbit anti-Myosin IIA (Sigma M8064) 1∶100, Rat Anti-Uvomorulin/E-cadherin (Sigma U3254) 1∶200, Rabbit anti-Dishevelled-2 (Biomol 4270) 1∶200, Sheep anti-Vangl2 (R&D Systems AF4815) 1∶100, and Rabbit anti-Par-3 (Millipore 07-330) 1∶100.

    Article Title: EPH Receptor Tyrosine Kinases Regulate Epithelial Morphogenesis and Phosphorylate the PAR-3 Scaffold Protein to Modulate Downstream Signaling Networks
    Article Snippet: Primary antibodies used were: rabbit anti-FLAG (Sigma-Aldrich, #F7425), mouse anti-pan phosphotyrosine 4G10 (Millipore, #05-321), mouse anti-GFP (Abcam, #290), mouse anti-EPHA4 (BD Biosciences, #610471), goat anti-EPHB2 (R&D Systems, # AF467), goat anti-EPHA1 (R&D Systems, #AF638), mouse anti-EPHA2 (Millipore, #05-480), goat anti-EPHB4 (R&D Systems, #AF3038), mouse anti-PKCι (BD, #610175), rabbit anti-CSK (Cell Signaling Technology, #4980), rabbit anti-PAR-3 (Millipore, #07-330), mouse anti-actin (Cell Signaling Technology, #3700).

    Article Title: Phosphorylation by Aurora kinase A facilitates cortical-cytoplasmic dynamics of Par-3 in asymmetric division of radial glia progenitors
    Article Snippet: Zebrafish embryos were treated with an AurkA inhibitor (MedChemExpress, MK-5108, HY-13252, CAS no. 1010085-13-8) from 22 to 24 hpf for various durations before imaging (10, 20, 30, 40, 50, 60, and 120 min).

    Article Title: EPH receptor tyrosine kinases phosphorylate the PAR-3 scaffold protein to modulate downstream signaling networks.
    Article Snippet: Primary antibodies used were: rabbit anti-FLAG (Sigma-Aldrich, #F7425), mouse anti-pan phosphotyrosine 4G10 (Sigma-Aldrich, #05–321), mouse anti-GFP (Thermo Fisher Scientific, #A-11122, Roche, #1181446001), mouse anti-EPHA4 (BD Biosciences, #610471), goat anti-EPHB2 (R&D Systems, # AF467), rabbit anti-CSK (Cell Signaling Technology, #4980), rabbit anti-PAR-3 (Millipore, #07–330), rabbit anti-LIMK2 (Cell Signaling Technology, #3845), rabbit anti-phospho-SRC (Tyr527) (Cell Signaling Technology, #2105), mouse anti-SRC (Cell Signaling Technology, #2110) mouse anti-Actin (Cell Signaling Technology, #3700), mouse anti-Tubulin (Cell Signaling Technology, #3873).

    Affinity Purification:


    Article Title: Polarized endosome dynamics engage cytosolic Par-3 and dynein during asymmetric division
    Article Snippet: Primary antibodies used in this study were: mouse anti-Dld (Abcam ab73331; RRID:AB_1268496; lot GR115501-3, 1:200 dilution for immunostaining) , chicken anti-GFP (Abcam ab13970; RRID:AB_300798, lot GR3190550-20, 1:500 dilution for immunostaining), rabbit anti-Par-3 (Millipore 07-330; RRID:AB_2101325; lot 3322358, 1:500 for immunostaining)(validated in this study), guinea pig anti-DLIC-Cter (a gift from D. T. Uemura, 1:100 for immunostaining and 1:500 for western blotting, validated in this study) , rabbit anti-α-Tubulin (Santa Cruz SC-12462; RRID:AB_2241125, lot A2907, 1:1000 for western blotting).

    Article Title: Nodal Dependent Differential Localisation of Dishevelled-2 Demarcates Regions of Differing Cell Behaviour in the Visceral Endoderm
    Article Snippet: Primary antibodies used were: Rabbit anti-ZO-1 (Zymed laboratories 61-7300) 1∶100, Rabbit anti-Myosin IIA (Sigma M8064) 1∶100, Rat Anti-Uvomorulin/E-cadherin (Sigma U3254) 1∶200, Rabbit anti-Dishevelled-2 (Biomol 4270) 1∶200, Sheep anti-Vangl2 (R&D Systems AF4815) 1∶100, and Rabbit anti-Par-3 (Millipore 07-330) 1∶100.

    Article Title: EPH Receptor Tyrosine Kinases Regulate Epithelial Morphogenesis and Phosphorylate the PAR-3 Scaffold Protein to Modulate Downstream Signaling Networks
    Article Snippet: Primary antibodies used were: rabbit anti-FLAG (Sigma-Aldrich, #F7425), mouse anti-pan phosphotyrosine 4G10 (Millipore, #05-321), mouse anti-GFP (Abcam, #290), mouse anti-EPHA4 (BD Biosciences, #610471), goat anti-EPHB2 (R&D Systems, # AF467), goat anti-EPHA1 (R&D Systems, #AF638), mouse anti-EPHA2 (Millipore, #05-480), goat anti-EPHB4 (R&D Systems, #AF3038), mouse anti-PKCι (BD, #610175), rabbit anti-CSK (Cell Signaling Technology, #4980), rabbit anti-PAR-3 (Millipore, #07-330), mouse anti-actin (Cell Signaling Technology, #3700).

    Article Title: Phosphorylation by Aurora kinase A facilitates cortical-cytoplasmic dynamics of Par-3 in asymmetric division of radial glia progenitors
    Article Snippet: Zebrafish embryos were treated with an AurkA inhibitor (MedChemExpress, MK-5108, HY-13252, CAS no. 1010085-13-8) from 22 to 24 hpf for various durations before imaging (10, 20, 30, 40, 50, 60, and 120 min).

    Article Title: EPH receptor tyrosine kinases phosphorylate the PAR-3 scaffold protein to modulate downstream signaling networks.
    Article Snippet: Primary antibodies used were: rabbit anti-FLAG (Sigma-Aldrich, #F7425), mouse anti-pan phosphotyrosine 4G10 (Sigma-Aldrich, #05–321), mouse anti-GFP (Thermo Fisher Scientific, #A-11122, Roche, #1181446001), mouse anti-EPHA4 (BD Biosciences, #610471), goat anti-EPHB2 (R&D Systems, # AF467), rabbit anti-CSK (Cell Signaling Technology, #4980), rabbit anti-PAR-3 (Millipore, #07–330), rabbit anti-LIMK2 (Cell Signaling Technology, #3845), rabbit anti-phospho-SRC (Tyr527) (Cell Signaling Technology, #2105), mouse anti-SRC (Cell Signaling Technology, #2110) mouse anti-Actin (Cell Signaling Technology, #3700), mouse anti-Tubulin (Cell Signaling Technology, #3873).

    In Vitro:


    Article Title: Polarized endosome dynamics engage cytosolic Par-3 and dynein during asymmetric division
    Article Snippet: Primary antibodies used in this study were: mouse anti-Dld (Abcam ab73331; RRID:AB_1268496; lot GR115501-3, 1:200 dilution for immunostaining) , chicken anti-GFP (Abcam ab13970; RRID:AB_300798, lot GR3190550-20, 1:500 dilution for immunostaining), rabbit anti-Par-3 (Millipore 07-330; RRID:AB_2101325; lot 3322358, 1:500 for immunostaining)(validated in this study), guinea pig anti-DLIC-Cter (a gift from D. T. Uemura, 1:100 for immunostaining and 1:500 for western blotting, validated in this study) , rabbit anti-α-Tubulin (Santa Cruz SC-12462; RRID:AB_2241125, lot A2907, 1:1000 for western blotting).

    Article Title: Nodal Dependent Differential Localisation of Dishevelled-2 Demarcates Regions of Differing Cell Behaviour in the Visceral Endoderm
    Article Snippet: Primary antibodies used were: Rabbit anti-ZO-1 (Zymed laboratories 61-7300) 1∶100, Rabbit anti-Myosin IIA (Sigma M8064) 1∶100, Rat Anti-Uvomorulin/E-cadherin (Sigma U3254) 1∶200, Rabbit anti-Dishevelled-2 (Biomol 4270) 1∶200, Sheep anti-Vangl2 (R&D Systems AF4815) 1∶100, and Rabbit anti-Par-3 (Millipore 07-330) 1∶100.

    Article Title: EPH Receptor Tyrosine Kinases Regulate Epithelial Morphogenesis and Phosphorylate the PAR-3 Scaffold Protein to Modulate Downstream Signaling Networks
    Article Snippet: Primary antibodies used were: rabbit anti-FLAG (Sigma-Aldrich, #F7425), mouse anti-pan phosphotyrosine 4G10 (Millipore, #05-321), mouse anti-GFP (Abcam, #290), mouse anti-EPHA4 (BD Biosciences, #610471), goat anti-EPHB2 (R&D Systems, # AF467), goat anti-EPHA1 (R&D Systems, #AF638), mouse anti-EPHA2 (Millipore, #05-480), goat anti-EPHB4 (R&D Systems, #AF3038), mouse anti-PKCι (BD, #610175), rabbit anti-CSK (Cell Signaling Technology, #4980), rabbit anti-PAR-3 (Millipore, #07-330), mouse anti-actin (Cell Signaling Technology, #3700).

    Article Title: Phosphorylation by Aurora kinase A facilitates cortical-cytoplasmic dynamics of Par-3 in asymmetric division of radial glia progenitors
    Article Snippet: Zebrafish embryos were treated with an AurkA inhibitor (MedChemExpress, MK-5108, HY-13252, CAS no. 1010085-13-8) from 22 to 24 hpf for various durations before imaging (10, 20, 30, 40, 50, 60, and 120 min).

    Article Title: EPH receptor tyrosine kinases phosphorylate the PAR-3 scaffold protein to modulate downstream signaling networks.
    Article Snippet: Primary antibodies used were: rabbit anti-FLAG (Sigma-Aldrich, #F7425), mouse anti-pan phosphotyrosine 4G10 (Sigma-Aldrich, #05–321), mouse anti-GFP (Thermo Fisher Scientific, #A-11122, Roche, #1181446001), mouse anti-EPHA4 (BD Biosciences, #610471), goat anti-EPHB2 (R&D Systems, # AF467), rabbit anti-CSK (Cell Signaling Technology, #4980), rabbit anti-PAR-3 (Millipore, #07–330), rabbit anti-LIMK2 (Cell Signaling Technology, #3845), rabbit anti-phospho-SRC (Tyr527) (Cell Signaling Technology, #2105), mouse anti-SRC (Cell Signaling Technology, #2110) mouse anti-Actin (Cell Signaling Technology, #3700), mouse anti-Tubulin (Cell Signaling Technology, #3873).

    Kinase Assay:


    Article Title: Polarized endosome dynamics engage cytosolic Par-3 and dynein during asymmetric division
    Article Snippet: Primary antibodies used in this study were: mouse anti-Dld (Abcam ab73331; RRID:AB_1268496; lot GR115501-3, 1:200 dilution for immunostaining) , chicken anti-GFP (Abcam ab13970; RRID:AB_300798, lot GR3190550-20, 1:500 dilution for immunostaining), rabbit anti-Par-3 (Millipore 07-330; RRID:AB_2101325; lot 3322358, 1:500 for immunostaining)(validated in this study), guinea pig anti-DLIC-Cter (a gift from D. T. Uemura, 1:100 for immunostaining and 1:500 for western blotting, validated in this study) , rabbit anti-α-Tubulin (Santa Cruz SC-12462; RRID:AB_2241125, lot A2907, 1:1000 for western blotting).

    Article Title: Nodal Dependent Differential Localisation of Dishevelled-2 Demarcates Regions of Differing Cell Behaviour in the Visceral Endoderm
    Article Snippet: Primary antibodies used were: Rabbit anti-ZO-1 (Zymed laboratories 61-7300) 1∶100, Rabbit anti-Myosin IIA (Sigma M8064) 1∶100, Rat Anti-Uvomorulin/E-cadherin (Sigma U3254) 1∶200, Rabbit anti-Dishevelled-2 (Biomol 4270) 1∶200, Sheep anti-Vangl2 (R&D Systems AF4815) 1∶100, and Rabbit anti-Par-3 (Millipore 07-330) 1∶100.

    Article Title: EPH Receptor Tyrosine Kinases Regulate Epithelial Morphogenesis and Phosphorylate the PAR-3 Scaffold Protein to Modulate Downstream Signaling Networks
    Article Snippet: Primary antibodies used were: rabbit anti-FLAG (Sigma-Aldrich, #F7425), mouse anti-pan phosphotyrosine 4G10 (Millipore, #05-321), mouse anti-GFP (Abcam, #290), mouse anti-EPHA4 (BD Biosciences, #610471), goat anti-EPHB2 (R&D Systems, # AF467), goat anti-EPHA1 (R&D Systems, #AF638), mouse anti-EPHA2 (Millipore, #05-480), goat anti-EPHB4 (R&D Systems, #AF3038), mouse anti-PKCι (BD, #610175), rabbit anti-CSK (Cell Signaling Technology, #4980), rabbit anti-PAR-3 (Millipore, #07-330), mouse anti-actin (Cell Signaling Technology, #3700).

    Article Title: Phosphorylation by Aurora kinase A facilitates cortical-cytoplasmic dynamics of Par-3 in asymmetric division of radial glia progenitors
    Article Snippet: Zebrafish embryos were treated with an AurkA inhibitor (MedChemExpress, MK-5108, HY-13252, CAS no. 1010085-13-8) from 22 to 24 hpf for various durations before imaging (10, 20, 30, 40, 50, 60, and 120 min).

    Article Title: EPH receptor tyrosine kinases phosphorylate the PAR-3 scaffold protein to modulate downstream signaling networks.
    Article Snippet: Primary antibodies used were: rabbit anti-FLAG (Sigma-Aldrich, #F7425), mouse anti-pan phosphotyrosine 4G10 (Sigma-Aldrich, #05–321), mouse anti-GFP (Thermo Fisher Scientific, #A-11122, Roche, #1181446001), mouse anti-EPHA4 (BD Biosciences, #610471), goat anti-EPHB2 (R&D Systems, # AF467), rabbit anti-CSK (Cell Signaling Technology, #4980), rabbit anti-PAR-3 (Millipore, #07–330), rabbit anti-LIMK2 (Cell Signaling Technology, #3845), rabbit anti-phospho-SRC (Tyr527) (Cell Signaling Technology, #2105), mouse anti-SRC (Cell Signaling Technology, #2110) mouse anti-Actin (Cell Signaling Technology, #3700), mouse anti-Tubulin (Cell Signaling Technology, #3873).

    Purification:


    Article Title: Polarized endosome dynamics engage cytosolic Par-3 and dynein during asymmetric division
    Article Snippet: Primary antibodies used in this study were: mouse anti-Dld (Abcam ab73331; RRID:AB_1268496; lot GR115501-3, 1:200 dilution for immunostaining) , chicken anti-GFP (Abcam ab13970; RRID:AB_300798, lot GR3190550-20, 1:500 dilution for immunostaining), rabbit anti-Par-3 (Millipore 07-330; RRID:AB_2101325; lot 3322358, 1:500 for immunostaining)(validated in this study), guinea pig anti-DLIC-Cter (a gift from D. T. Uemura, 1:100 for immunostaining and 1:500 for western blotting, validated in this study) , rabbit anti-α-Tubulin (Santa Cruz SC-12462; RRID:AB_2241125, lot A2907, 1:1000 for western blotting).

    Article Title: Nodal Dependent Differential Localisation of Dishevelled-2 Demarcates Regions of Differing Cell Behaviour in the Visceral Endoderm
    Article Snippet: Primary antibodies used were: Rabbit anti-ZO-1 (Zymed laboratories 61-7300) 1∶100, Rabbit anti-Myosin IIA (Sigma M8064) 1∶100, Rat Anti-Uvomorulin/E-cadherin (Sigma U3254) 1∶200, Rabbit anti-Dishevelled-2 (Biomol 4270) 1∶200, Sheep anti-Vangl2 (R&D Systems AF4815) 1∶100, and Rabbit anti-Par-3 (Millipore 07-330) 1∶100.

    Article Title: EPH Receptor Tyrosine Kinases Regulate Epithelial Morphogenesis and Phosphorylate the PAR-3 Scaffold Protein to Modulate Downstream Signaling Networks
    Article Snippet: Primary antibodies used were: rabbit anti-FLAG (Sigma-Aldrich, #F7425), mouse anti-pan phosphotyrosine 4G10 (Millipore, #05-321), mouse anti-GFP (Abcam, #290), mouse anti-EPHA4 (BD Biosciences, #610471), goat anti-EPHB2 (R&D Systems, # AF467), goat anti-EPHA1 (R&D Systems, #AF638), mouse anti-EPHA2 (Millipore, #05-480), goat anti-EPHB4 (R&D Systems, #AF3038), mouse anti-PKCι (BD, #610175), rabbit anti-CSK (Cell Signaling Technology, #4980), rabbit anti-PAR-3 (Millipore, #07-330), mouse anti-actin (Cell Signaling Technology, #3700).

    Article Title: Phosphorylation by Aurora kinase A facilitates cortical-cytoplasmic dynamics of Par-3 in asymmetric division of radial glia progenitors
    Article Snippet: Zebrafish embryos were treated with an AurkA inhibitor (MedChemExpress, MK-5108, HY-13252, CAS no. 1010085-13-8) from 22 to 24 hpf for various durations before imaging (10, 20, 30, 40, 50, 60, and 120 min).

    Article Title: EPH receptor tyrosine kinases phosphorylate the PAR-3 scaffold protein to modulate downstream signaling networks.
    Article Snippet: Primary antibodies used were: rabbit anti-FLAG (Sigma-Aldrich, #F7425), mouse anti-pan phosphotyrosine 4G10 (Sigma-Aldrich, #05–321), mouse anti-GFP (Thermo Fisher Scientific, #A-11122, Roche, #1181446001), mouse anti-EPHA4 (BD Biosciences, #610471), goat anti-EPHB2 (R&D Systems, # AF467), rabbit anti-CSK (Cell Signaling Technology, #4980), rabbit anti-PAR-3 (Millipore, #07–330), rabbit anti-LIMK2 (Cell Signaling Technology, #3845), rabbit anti-phospho-SRC (Tyr527) (Cell Signaling Technology, #2105), mouse anti-SRC (Cell Signaling Technology, #2110) mouse anti-Actin (Cell Signaling Technology, #3700), mouse anti-Tubulin (Cell Signaling Technology, #3873).

    Recombinant:


    Article Title: Polarized endosome dynamics engage cytosolic Par-3 and dynein during asymmetric division
    Article Snippet: Primary antibodies used in this study were: mouse anti-Dld (Abcam ab73331; RRID:AB_1268496; lot GR115501-3, 1:200 dilution for immunostaining) , chicken anti-GFP (Abcam ab13970; RRID:AB_300798, lot GR3190550-20, 1:500 dilution for immunostaining), rabbit anti-Par-3 (Millipore 07-330; RRID:AB_2101325; lot 3322358, 1:500 for immunostaining)(validated in this study), guinea pig anti-DLIC-Cter (a gift from D. T. Uemura, 1:100 for immunostaining and 1:500 for western blotting, validated in this study) , rabbit anti-α-Tubulin (Santa Cruz SC-12462; RRID:AB_2241125, lot A2907, 1:1000 for western blotting).

    Article Title: Nodal Dependent Differential Localisation of Dishevelled-2 Demarcates Regions of Differing Cell Behaviour in the Visceral Endoderm
    Article Snippet: Primary antibodies used were: Rabbit anti-ZO-1 (Zymed laboratories 61-7300) 1∶100, Rabbit anti-Myosin IIA (Sigma M8064) 1∶100, Rat Anti-Uvomorulin/E-cadherin (Sigma U3254) 1∶200, Rabbit anti-Dishevelled-2 (Biomol 4270) 1∶200, Sheep anti-Vangl2 (R&D Systems AF4815) 1∶100, and Rabbit anti-Par-3 (Millipore 07-330) 1∶100.

    Article Title: EPH Receptor Tyrosine Kinases Regulate Epithelial Morphogenesis and Phosphorylate the PAR-3 Scaffold Protein to Modulate Downstream Signaling Networks
    Article Snippet: Primary antibodies used were: rabbit anti-FLAG (Sigma-Aldrich, #F7425), mouse anti-pan phosphotyrosine 4G10 (Millipore, #05-321), mouse anti-GFP (Abcam, #290), mouse anti-EPHA4 (BD Biosciences, #610471), goat anti-EPHB2 (R&D Systems, # AF467), goat anti-EPHA1 (R&D Systems, #AF638), mouse anti-EPHA2 (Millipore, #05-480), goat anti-EPHB4 (R&D Systems, #AF3038), mouse anti-PKCι (BD, #610175), rabbit anti-CSK (Cell Signaling Technology, #4980), rabbit anti-PAR-3 (Millipore, #07-330), mouse anti-actin (Cell Signaling Technology, #3700).

    Article Title: Phosphorylation by Aurora kinase A facilitates cortical-cytoplasmic dynamics of Par-3 in asymmetric division of radial glia progenitors
    Article Snippet: Zebrafish embryos were treated with an AurkA inhibitor (MedChemExpress, MK-5108, HY-13252, CAS no. 1010085-13-8) from 22 to 24 hpf for various durations before imaging (10, 20, 30, 40, 50, 60, and 120 min).

    Article Title: EPH receptor tyrosine kinases phosphorylate the PAR-3 scaffold protein to modulate downstream signaling networks.
    Article Snippet: Primary antibodies used were: rabbit anti-FLAG (Sigma-Aldrich, #F7425), mouse anti-pan phosphotyrosine 4G10 (Sigma-Aldrich, #05–321), mouse anti-GFP (Thermo Fisher Scientific, #A-11122, Roche, #1181446001), mouse anti-EPHA4 (BD Biosciences, #610471), goat anti-EPHB2 (R&D Systems, # AF467), rabbit anti-CSK (Cell Signaling Technology, #4980), rabbit anti-PAR-3 (Millipore, #07–330), rabbit anti-LIMK2 (Cell Signaling Technology, #3845), rabbit anti-phospho-SRC (Tyr527) (Cell Signaling Technology, #2105), mouse anti-SRC (Cell Signaling Technology, #2110) mouse anti-Actin (Cell Signaling Technology, #3700), mouse anti-Tubulin (Cell Signaling Technology, #3873).

    SDS Page:


    Article Title: Polarized endosome dynamics engage cytosolic Par-3 and dynein during asymmetric division
    Article Snippet: Primary antibodies used in this study were: mouse anti-Dld (Abcam ab73331; RRID:AB_1268496; lot GR115501-3, 1:200 dilution for immunostaining) , chicken anti-GFP (Abcam ab13970; RRID:AB_300798, lot GR3190550-20, 1:500 dilution for immunostaining), rabbit anti-Par-3 (Millipore 07-330; RRID:AB_2101325; lot 3322358, 1:500 for immunostaining)(validated in this study), guinea pig anti-DLIC-Cter (a gift from D. T. Uemura, 1:100 for immunostaining and 1:500 for western blotting, validated in this study) , rabbit anti-α-Tubulin (Santa Cruz SC-12462; RRID:AB_2241125, lot A2907, 1:1000 for western blotting).

    Article Title: Nodal Dependent Differential Localisation of Dishevelled-2 Demarcates Regions of Differing Cell Behaviour in the Visceral Endoderm
    Article Snippet: Primary antibodies used were: Rabbit anti-ZO-1 (Zymed laboratories 61-7300) 1∶100, Rabbit anti-Myosin IIA (Sigma M8064) 1∶100, Rat Anti-Uvomorulin/E-cadherin (Sigma U3254) 1∶200, Rabbit anti-Dishevelled-2 (Biomol 4270) 1∶200, Sheep anti-Vangl2 (R&D Systems AF4815) 1∶100, and Rabbit anti-Par-3 (Millipore 07-330) 1∶100.

    Article Title: EPH Receptor Tyrosine Kinases Regulate Epithelial Morphogenesis and Phosphorylate the PAR-3 Scaffold Protein to Modulate Downstream Signaling Networks
    Article Snippet: Primary antibodies used were: rabbit anti-FLAG (Sigma-Aldrich, #F7425), mouse anti-pan phosphotyrosine 4G10 (Millipore, #05-321), mouse anti-GFP (Abcam, #290), mouse anti-EPHA4 (BD Biosciences, #610471), goat anti-EPHB2 (R&D Systems, # AF467), goat anti-EPHA1 (R&D Systems, #AF638), mouse anti-EPHA2 (Millipore, #05-480), goat anti-EPHB4 (R&D Systems, #AF3038), mouse anti-PKCι (BD, #610175), rabbit anti-CSK (Cell Signaling Technology, #4980), rabbit anti-PAR-3 (Millipore, #07-330), mouse anti-actin (Cell Signaling Technology, #3700).

    Article Title: Phosphorylation by Aurora kinase A facilitates cortical-cytoplasmic dynamics of Par-3 in asymmetric division of radial glia progenitors
    Article Snippet: Zebrafish embryos were treated with an AurkA inhibitor (MedChemExpress, MK-5108, HY-13252, CAS no. 1010085-13-8) from 22 to 24 hpf for various durations before imaging (10, 20, 30, 40, 50, 60, and 120 min).

    Article Title: EPH receptor tyrosine kinases phosphorylate the PAR-3 scaffold protein to modulate downstream signaling networks.
    Article Snippet: Primary antibodies used were: rabbit anti-FLAG (Sigma-Aldrich, #F7425), mouse anti-pan phosphotyrosine 4G10 (Sigma-Aldrich, #05–321), mouse anti-GFP (Thermo Fisher Scientific, #A-11122, Roche, #1181446001), mouse anti-EPHA4 (BD Biosciences, #610471), goat anti-EPHB2 (R&D Systems, # AF467), rabbit anti-CSK (Cell Signaling Technology, #4980), rabbit anti-PAR-3 (Millipore, #07–330), rabbit anti-LIMK2 (Cell Signaling Technology, #3845), rabbit anti-phospho-SRC (Tyr527) (Cell Signaling Technology, #2105), mouse anti-SRC (Cell Signaling Technology, #2110) mouse anti-Actin (Cell Signaling Technology, #3700), mouse anti-Tubulin (Cell Signaling Technology, #3873).

    Autoradiography:


    Article Title: Polarized endosome dynamics engage cytosolic Par-3 and dynein during asymmetric division
    Article Snippet: Primary antibodies used in this study were: mouse anti-Dld (Abcam ab73331; RRID:AB_1268496; lot GR115501-3, 1:200 dilution for immunostaining) , chicken anti-GFP (Abcam ab13970; RRID:AB_300798, lot GR3190550-20, 1:500 dilution for immunostaining), rabbit anti-Par-3 (Millipore 07-330; RRID:AB_2101325; lot 3322358, 1:500 for immunostaining)(validated in this study), guinea pig anti-DLIC-Cter (a gift from D. T. Uemura, 1:100 for immunostaining and 1:500 for western blotting, validated in this study) , rabbit anti-α-Tubulin (Santa Cruz SC-12462; RRID:AB_2241125, lot A2907, 1:1000 for western blotting).

    Article Title: Nodal Dependent Differential Localisation of Dishevelled-2 Demarcates Regions of Differing Cell Behaviour in the Visceral Endoderm
    Article Snippet: Primary antibodies used were: Rabbit anti-ZO-1 (Zymed laboratories 61-7300) 1∶100, Rabbit anti-Myosin IIA (Sigma M8064) 1∶100, Rat Anti-Uvomorulin/E-cadherin (Sigma U3254) 1∶200, Rabbit anti-Dishevelled-2 (Biomol 4270) 1∶200, Sheep anti-Vangl2 (R&D Systems AF4815) 1∶100, and Rabbit anti-Par-3 (Millipore 07-330) 1∶100.

    Article Title: EPH Receptor Tyrosine Kinases Regulate Epithelial Morphogenesis and Phosphorylate the PAR-3 Scaffold Protein to Modulate Downstream Signaling Networks
    Article Snippet: Primary antibodies used were: rabbit anti-FLAG (Sigma-Aldrich, #F7425), mouse anti-pan phosphotyrosine 4G10 (Millipore, #05-321), mouse anti-GFP (Abcam, #290), mouse anti-EPHA4 (BD Biosciences, #610471), goat anti-EPHB2 (R&D Systems, # AF467), goat anti-EPHA1 (R&D Systems, #AF638), mouse anti-EPHA2 (Millipore, #05-480), goat anti-EPHB4 (R&D Systems, #AF3038), mouse anti-PKCι (BD, #610175), rabbit anti-CSK (Cell Signaling Technology, #4980), rabbit anti-PAR-3 (Millipore, #07-330), mouse anti-actin (Cell Signaling Technology, #3700).

    Article Title: Phosphorylation by Aurora kinase A facilitates cortical-cytoplasmic dynamics of Par-3 in asymmetric division of radial glia progenitors
    Article Snippet: Zebrafish embryos were treated with an AurkA inhibitor (MedChemExpress, MK-5108, HY-13252, CAS no. 1010085-13-8) from 22 to 24 hpf for various durations before imaging (10, 20, 30, 40, 50, 60, and 120 min).

    Article Title: EPH receptor tyrosine kinases phosphorylate the PAR-3 scaffold protein to modulate downstream signaling networks.
    Article Snippet: Primary antibodies used were: rabbit anti-FLAG (Sigma-Aldrich, #F7425), mouse anti-pan phosphotyrosine 4G10 (Sigma-Aldrich, #05–321), mouse anti-GFP (Thermo Fisher Scientific, #A-11122, Roche, #1181446001), mouse anti-EPHA4 (BD Biosciences, #610471), goat anti-EPHB2 (R&D Systems, # AF467), rabbit anti-CSK (Cell Signaling Technology, #4980), rabbit anti-PAR-3 (Millipore, #07–330), rabbit anti-LIMK2 (Cell Signaling Technology, #3845), rabbit anti-phospho-SRC (Tyr527) (Cell Signaling Technology, #2105), mouse anti-SRC (Cell Signaling Technology, #2110) mouse anti-Actin (Cell Signaling Technology, #3700), mouse anti-Tubulin (Cell Signaling Technology, #3873).

    Binding Assay:


    Article Title: Polarized endosome dynamics engage cytosolic Par-3 and dynein during asymmetric division
    Article Snippet: Primary antibodies used in this study were: mouse anti-Dld (Abcam ab73331; RRID:AB_1268496; lot GR115501-3, 1:200 dilution for immunostaining) , chicken anti-GFP (Abcam ab13970; RRID:AB_300798, lot GR3190550-20, 1:500 dilution for immunostaining), rabbit anti-Par-3 (Millipore 07-330; RRID:AB_2101325; lot 3322358, 1:500 for immunostaining)(validated in this study), guinea pig anti-DLIC-Cter (a gift from D. T. Uemura, 1:100 for immunostaining and 1:500 for western blotting, validated in this study) , rabbit anti-α-Tubulin (Santa Cruz SC-12462; RRID:AB_2241125, lot A2907, 1:1000 for western blotting).

    Article Title: Nodal Dependent Differential Localisation of Dishevelled-2 Demarcates Regions of Differing Cell Behaviour in the Visceral Endoderm
    Article Snippet: Primary antibodies used were: Rabbit anti-ZO-1 (Zymed laboratories 61-7300) 1∶100, Rabbit anti-Myosin IIA (Sigma M8064) 1∶100, Rat Anti-Uvomorulin/E-cadherin (Sigma U3254) 1∶200, Rabbit anti-Dishevelled-2 (Biomol 4270) 1∶200, Sheep anti-Vangl2 (R&D Systems AF4815) 1∶100, and Rabbit anti-Par-3 (Millipore 07-330) 1∶100.

    Article Title: EPH Receptor Tyrosine Kinases Regulate Epithelial Morphogenesis and Phosphorylate the PAR-3 Scaffold Protein to Modulate Downstream Signaling Networks
    Article Snippet: Primary antibodies used were: rabbit anti-FLAG (Sigma-Aldrich, #F7425), mouse anti-pan phosphotyrosine 4G10 (Millipore, #05-321), mouse anti-GFP (Abcam, #290), mouse anti-EPHA4 (BD Biosciences, #610471), goat anti-EPHB2 (R&D Systems, # AF467), goat anti-EPHA1 (R&D Systems, #AF638), mouse anti-EPHA2 (Millipore, #05-480), goat anti-EPHB4 (R&D Systems, #AF3038), mouse anti-PKCι (BD, #610175), rabbit anti-CSK (Cell Signaling Technology, #4980), rabbit anti-PAR-3 (Millipore, #07-330), mouse anti-actin (Cell Signaling Technology, #3700).

    Article Title: Phosphorylation by Aurora kinase A facilitates cortical-cytoplasmic dynamics of Par-3 in asymmetric division of radial glia progenitors
    Article Snippet: Zebrafish embryos were treated with an AurkA inhibitor (MedChemExpress, MK-5108, HY-13252, CAS no. 1010085-13-8) from 22 to 24 hpf for various durations before imaging (10, 20, 30, 40, 50, 60, and 120 min).

    Article Title: EPH receptor tyrosine kinases phosphorylate the PAR-3 scaffold protein to modulate downstream signaling networks.
    Article Snippet: Primary antibodies used were: rabbit anti-FLAG (Sigma-Aldrich, #F7425), mouse anti-pan phosphotyrosine 4G10 (Sigma-Aldrich, #05–321), mouse anti-GFP (Thermo Fisher Scientific, #A-11122, Roche, #1181446001), mouse anti-EPHA4 (BD Biosciences, #610471), goat anti-EPHB2 (R&D Systems, # AF467), rabbit anti-CSK (Cell Signaling Technology, #4980), rabbit anti-PAR-3 (Millipore, #07–330), rabbit anti-LIMK2 (Cell Signaling Technology, #3845), rabbit anti-phospho-SRC (Tyr527) (Cell Signaling Technology, #2105), mouse anti-SRC (Cell Signaling Technology, #2110) mouse anti-Actin (Cell Signaling Technology, #3700), mouse anti-Tubulin (Cell Signaling Technology, #3873).

    Synthesized:


    Article Title: Polarized endosome dynamics engage cytosolic Par-3 and dynein during asymmetric division
    Article Snippet: Primary antibodies used in this study were: mouse anti-Dld (Abcam ab73331; RRID:AB_1268496; lot GR115501-3, 1:200 dilution for immunostaining) , chicken anti-GFP (Abcam ab13970; RRID:AB_300798, lot GR3190550-20, 1:500 dilution for immunostaining), rabbit anti-Par-3 (Millipore 07-330; RRID:AB_2101325; lot 3322358, 1:500 for immunostaining)(validated in this study), guinea pig anti-DLIC-Cter (a gift from D. T. Uemura, 1:100 for immunostaining and 1:500 for western blotting, validated in this study) , rabbit anti-α-Tubulin (Santa Cruz SC-12462; RRID:AB_2241125, lot A2907, 1:1000 for western blotting).

    Article Title: Nodal Dependent Differential Localisation of Dishevelled-2 Demarcates Regions of Differing Cell Behaviour in the Visceral Endoderm
    Article Snippet: Primary antibodies used were: Rabbit anti-ZO-1 (Zymed laboratories 61-7300) 1∶100, Rabbit anti-Myosin IIA (Sigma M8064) 1∶100, Rat Anti-Uvomorulin/E-cadherin (Sigma U3254) 1∶200, Rabbit anti-Dishevelled-2 (Biomol 4270) 1∶200, Sheep anti-Vangl2 (R&D Systems AF4815) 1∶100, and Rabbit anti-Par-3 (Millipore 07-330) 1∶100.

    Article Title: EPH Receptor Tyrosine Kinases Regulate Epithelial Morphogenesis and Phosphorylate the PAR-3 Scaffold Protein to Modulate Downstream Signaling Networks
    Article Snippet: Primary antibodies used were: rabbit anti-FLAG (Sigma-Aldrich, #F7425), mouse anti-pan phosphotyrosine 4G10 (Millipore, #05-321), mouse anti-GFP (Abcam, #290), mouse anti-EPHA4 (BD Biosciences, #610471), goat anti-EPHB2 (R&D Systems, # AF467), goat anti-EPHA1 (R&D Systems, #AF638), mouse anti-EPHA2 (Millipore, #05-480), goat anti-EPHB4 (R&D Systems, #AF3038), mouse anti-PKCι (BD, #610175), rabbit anti-CSK (Cell Signaling Technology, #4980), rabbit anti-PAR-3 (Millipore, #07-330), mouse anti-actin (Cell Signaling Technology, #3700).

    Article Title: Phosphorylation by Aurora kinase A facilitates cortical-cytoplasmic dynamics of Par-3 in asymmetric division of radial glia progenitors
    Article Snippet: Zebrafish embryos were treated with an AurkA inhibitor (MedChemExpress, MK-5108, HY-13252, CAS no. 1010085-13-8) from 22 to 24 hpf for various durations before imaging (10, 20, 30, 40, 50, 60, and 120 min).

    Article Title: EPH receptor tyrosine kinases phosphorylate the PAR-3 scaffold protein to modulate downstream signaling networks.
    Article Snippet: Primary antibodies used were: rabbit anti-FLAG (Sigma-Aldrich, #F7425), mouse anti-pan phosphotyrosine 4G10 (Sigma-Aldrich, #05–321), mouse anti-GFP (Thermo Fisher Scientific, #A-11122, Roche, #1181446001), mouse anti-EPHA4 (BD Biosciences, #610471), goat anti-EPHB2 (R&D Systems, # AF467), rabbit anti-CSK (Cell Signaling Technology, #4980), rabbit anti-PAR-3 (Millipore, #07–330), rabbit anti-LIMK2 (Cell Signaling Technology, #3845), rabbit anti-phospho-SRC (Tyr527) (Cell Signaling Technology, #2105), mouse anti-SRC (Cell Signaling Technology, #2110) mouse anti-Actin (Cell Signaling Technology, #3700), mouse anti-Tubulin (Cell Signaling Technology, #3873).

    Incubation:


    Article Title: Polarized endosome dynamics engage cytosolic Par-3 and dynein during asymmetric division
    Article Snippet: Primary antibodies used in this study were: mouse anti-Dld (Abcam ab73331; RRID:AB_1268496; lot GR115501-3, 1:200 dilution for immunostaining) , chicken anti-GFP (Abcam ab13970; RRID:AB_300798, lot GR3190550-20, 1:500 dilution for immunostaining), rabbit anti-Par-3 (Millipore 07-330; RRID:AB_2101325; lot 3322358, 1:500 for immunostaining)(validated in this study), guinea pig anti-DLIC-Cter (a gift from D. T. Uemura, 1:100 for immunostaining and 1:500 for western blotting, validated in this study) , rabbit anti-α-Tubulin (Santa Cruz SC-12462; RRID:AB_2241125, lot A2907, 1:1000 for western blotting).

    Article Title: Nodal Dependent Differential Localisation of Dishevelled-2 Demarcates Regions of Differing Cell Behaviour in the Visceral Endoderm
    Article Snippet: Primary antibodies used were: Rabbit anti-ZO-1 (Zymed laboratories 61-7300) 1∶100, Rabbit anti-Myosin IIA (Sigma M8064) 1∶100, Rat Anti-Uvomorulin/E-cadherin (Sigma U3254) 1∶200, Rabbit anti-Dishevelled-2 (Biomol 4270) 1∶200, Sheep anti-Vangl2 (R&D Systems AF4815) 1∶100, and Rabbit anti-Par-3 (Millipore 07-330) 1∶100.

    Article Title: EPH Receptor Tyrosine Kinases Regulate Epithelial Morphogenesis and Phosphorylate the PAR-3 Scaffold Protein to Modulate Downstream Signaling Networks
    Article Snippet: Primary antibodies used were: rabbit anti-FLAG (Sigma-Aldrich, #F7425), mouse anti-pan phosphotyrosine 4G10 (Millipore, #05-321), mouse anti-GFP (Abcam, #290), mouse anti-EPHA4 (BD Biosciences, #610471), goat anti-EPHB2 (R&D Systems, # AF467), goat anti-EPHA1 (R&D Systems, #AF638), mouse anti-EPHA2 (Millipore, #05-480), goat anti-EPHB4 (R&D Systems, #AF3038), mouse anti-PKCι (BD, #610175), rabbit anti-CSK (Cell Signaling Technology, #4980), rabbit anti-PAR-3 (Millipore, #07-330), mouse anti-actin (Cell Signaling Technology, #3700).

    Article Title: Phosphorylation by Aurora kinase A facilitates cortical-cytoplasmic dynamics of Par-3 in asymmetric division of radial glia progenitors
    Article Snippet: Zebrafish embryos were treated with an AurkA inhibitor (MedChemExpress, MK-5108, HY-13252, CAS no. 1010085-13-8) from 22 to 24 hpf for various durations before imaging (10, 20, 30, 40, 50, 60, and 120 min).

    Article Title: EPH receptor tyrosine kinases phosphorylate the PAR-3 scaffold protein to modulate downstream signaling networks.
    Article Snippet: Primary antibodies used were: rabbit anti-FLAG (Sigma-Aldrich, #F7425), mouse anti-pan phosphotyrosine 4G10 (Sigma-Aldrich, #05–321), mouse anti-GFP (Thermo Fisher Scientific, #A-11122, Roche, #1181446001), mouse anti-EPHA4 (BD Biosciences, #610471), goat anti-EPHB2 (R&D Systems, # AF467), rabbit anti-CSK (Cell Signaling Technology, #4980), rabbit anti-PAR-3 (Millipore, #07–330), rabbit anti-LIMK2 (Cell Signaling Technology, #3845), rabbit anti-phospho-SRC (Tyr527) (Cell Signaling Technology, #2105), mouse anti-SRC (Cell Signaling Technology, #2110) mouse anti-Actin (Cell Signaling Technology, #3700), mouse anti-Tubulin (Cell Signaling Technology, #3873).

    Transfection:


    Article Title: Polarized endosome dynamics engage cytosolic Par-3 and dynein during asymmetric division
    Article Snippet: Primary antibodies used in this study were: mouse anti-Dld (Abcam ab73331; RRID:AB_1268496; lot GR115501-3, 1:200 dilution for immunostaining) , chicken anti-GFP (Abcam ab13970; RRID:AB_300798, lot GR3190550-20, 1:500 dilution for immunostaining), rabbit anti-Par-3 (Millipore 07-330; RRID:AB_2101325; lot 3322358, 1:500 for immunostaining)(validated in this study), guinea pig anti-DLIC-Cter (a gift from D. T. Uemura, 1:100 for immunostaining and 1:500 for western blotting, validated in this study) , rabbit anti-α-Tubulin (Santa Cruz SC-12462; RRID:AB_2241125, lot A2907, 1:1000 for western blotting).

    Article Title: Nodal Dependent Differential Localisation of Dishevelled-2 Demarcates Regions of Differing Cell Behaviour in the Visceral Endoderm
    Article Snippet: Primary antibodies used were: Rabbit anti-ZO-1 (Zymed laboratories 61-7300) 1∶100, Rabbit anti-Myosin IIA (Sigma M8064) 1∶100, Rat Anti-Uvomorulin/E-cadherin (Sigma U3254) 1∶200, Rabbit anti-Dishevelled-2 (Biomol 4270) 1∶200, Sheep anti-Vangl2 (R&D Systems AF4815) 1∶100, and Rabbit anti-Par-3 (Millipore 07-330) 1∶100.

    Article Title: EPH Receptor Tyrosine Kinases Regulate Epithelial Morphogenesis and Phosphorylate the PAR-3 Scaffold Protein to Modulate Downstream Signaling Networks
    Article Snippet: Primary antibodies used were: rabbit anti-FLAG (Sigma-Aldrich, #F7425), mouse anti-pan phosphotyrosine 4G10 (Millipore, #05-321), mouse anti-GFP (Abcam, #290), mouse anti-EPHA4 (BD Biosciences, #610471), goat anti-EPHB2 (R&D Systems, # AF467), goat anti-EPHA1 (R&D Systems, #AF638), mouse anti-EPHA2 (Millipore, #05-480), goat anti-EPHB4 (R&D Systems, #AF3038), mouse anti-PKCι (BD, #610175), rabbit anti-CSK (Cell Signaling Technology, #4980), rabbit anti-PAR-3 (Millipore, #07-330), mouse anti-actin (Cell Signaling Technology, #3700).

    Article Title: Phosphorylation by Aurora kinase A facilitates cortical-cytoplasmic dynamics of Par-3 in asymmetric division of radial glia progenitors
    Article Snippet: Zebrafish embryos were treated with an AurkA inhibitor (MedChemExpress, MK-5108, HY-13252, CAS no. 1010085-13-8) from 22 to 24 hpf for various durations before imaging (10, 20, 30, 40, 50, 60, and 120 min).

    Article Title: EPH receptor tyrosine kinases phosphorylate the PAR-3 scaffold protein to modulate downstream signaling networks.
    Article Snippet: Primary antibodies used were: rabbit anti-FLAG (Sigma-Aldrich, #F7425), mouse anti-pan phosphotyrosine 4G10 (Sigma-Aldrich, #05–321), mouse anti-GFP (Thermo Fisher Scientific, #A-11122, Roche, #1181446001), mouse anti-EPHA4 (BD Biosciences, #610471), goat anti-EPHB2 (R&D Systems, # AF467), rabbit anti-CSK (Cell Signaling Technology, #4980), rabbit anti-PAR-3 (Millipore, #07–330), rabbit anti-LIMK2 (Cell Signaling Technology, #3845), rabbit anti-phospho-SRC (Tyr527) (Cell Signaling Technology, #2105), mouse anti-SRC (Cell Signaling Technology, #2110) mouse anti-Actin (Cell Signaling Technology, #3700), mouse anti-Tubulin (Cell Signaling Technology, #3873).

    Immunostaining:


    Article Title: Polarized endosome dynamics engage cytosolic Par-3 and dynein during asymmetric division
    Article Snippet: Primary antibodies used in this study were: mouse anti-Dld (Abcam ab73331; RRID:AB_1268496; lot GR115501-3, 1:200 dilution for immunostaining) , chicken anti-GFP (Abcam ab13970; RRID:AB_300798, lot GR3190550-20, 1:500 dilution for immunostaining), rabbit anti-Par-3 (Millipore 07-330; RRID:AB_2101325; lot 3322358, 1:500 for immunostaining)(validated in this study), guinea pig anti-DLIC-Cter (a gift from D. T. Uemura, 1:100 for immunostaining and 1:500 for western blotting, validated in this study) , rabbit anti-α-Tubulin (Santa Cruz SC-12462; RRID:AB_2241125, lot A2907, 1:1000 for western blotting).

    Article Title: Nodal Dependent Differential Localisation of Dishevelled-2 Demarcates Regions of Differing Cell Behaviour in the Visceral Endoderm
    Article Snippet: Primary antibodies used were: Rabbit anti-ZO-1 (Zymed laboratories 61-7300) 1∶100, Rabbit anti-Myosin IIA (Sigma M8064) 1∶100, Rat Anti-Uvomorulin/E-cadherin (Sigma U3254) 1∶200, Rabbit anti-Dishevelled-2 (Biomol 4270) 1∶200, Sheep anti-Vangl2 (R&D Systems AF4815) 1∶100, and Rabbit anti-Par-3 (Millipore 07-330) 1∶100.

    Article Title: EPH Receptor Tyrosine Kinases Regulate Epithelial Morphogenesis and Phosphorylate the PAR-3 Scaffold Protein to Modulate Downstream Signaling Networks
    Article Snippet: Primary antibodies used were: rabbit anti-FLAG (Sigma-Aldrich, #F7425), mouse anti-pan phosphotyrosine 4G10 (Millipore, #05-321), mouse anti-GFP (Abcam, #290), mouse anti-EPHA4 (BD Biosciences, #610471), goat anti-EPHB2 (R&D Systems, # AF467), goat anti-EPHA1 (R&D Systems, #AF638), mouse anti-EPHA2 (Millipore, #05-480), goat anti-EPHB4 (R&D Systems, #AF3038), mouse anti-PKCι (BD, #610175), rabbit anti-CSK (Cell Signaling Technology, #4980), rabbit anti-PAR-3 (Millipore, #07-330), mouse anti-actin (Cell Signaling Technology, #3700).

    Article Title: Phosphorylation by Aurora kinase A facilitates cortical-cytoplasmic dynamics of Par-3 in asymmetric division of radial glia progenitors
    Article Snippet: Zebrafish embryos were treated with an AurkA inhibitor (MedChemExpress, MK-5108, HY-13252, CAS no. 1010085-13-8) from 22 to 24 hpf for various durations before imaging (10, 20, 30, 40, 50, 60, and 120 min).

    Article Title: EPH receptor tyrosine kinases phosphorylate the PAR-3 scaffold protein to modulate downstream signaling networks.
    Article Snippet: Primary antibodies used were: rabbit anti-FLAG (Sigma-Aldrich, #F7425), mouse anti-pan phosphotyrosine 4G10 (Sigma-Aldrich, #05–321), mouse anti-GFP (Thermo Fisher Scientific, #A-11122, Roche, #1181446001), mouse anti-EPHA4 (BD Biosciences, #610471), goat anti-EPHB2 (R&D Systems, # AF467), rabbit anti-CSK (Cell Signaling Technology, #4980), rabbit anti-PAR-3 (Millipore, #07–330), rabbit anti-LIMK2 (Cell Signaling Technology, #3845), rabbit anti-phospho-SRC (Tyr527) (Cell Signaling Technology, #2105), mouse anti-SRC (Cell Signaling Technology, #2110) mouse anti-Actin (Cell Signaling Technology, #3700), mouse anti-Tubulin (Cell Signaling Technology, #3873).

    Sequencing:


    Article Title: Polarized endosome dynamics engage cytosolic Par-3 and dynein during asymmetric division
    Article Snippet: Primary antibodies used in this study were: mouse anti-Dld (Abcam ab73331; RRID:AB_1268496; lot GR115501-3, 1:200 dilution for immunostaining) , chicken anti-GFP (Abcam ab13970; RRID:AB_300798, lot GR3190550-20, 1:500 dilution for immunostaining), rabbit anti-Par-3 (Millipore 07-330; RRID:AB_2101325; lot 3322358, 1:500 for immunostaining)(validated in this study), guinea pig anti-DLIC-Cter (a gift from D. T. Uemura, 1:100 for immunostaining and 1:500 for western blotting, validated in this study) , rabbit anti-α-Tubulin (Santa Cruz SC-12462; RRID:AB_2241125, lot A2907, 1:1000 for western blotting).

    Article Title: Nodal Dependent Differential Localisation of Dishevelled-2 Demarcates Regions of Differing Cell Behaviour in the Visceral Endoderm
    Article Snippet: Primary antibodies used were: Rabbit anti-ZO-1 (Zymed laboratories 61-7300) 1∶100, Rabbit anti-Myosin IIA (Sigma M8064) 1∶100, Rat Anti-Uvomorulin/E-cadherin (Sigma U3254) 1∶200, Rabbit anti-Dishevelled-2 (Biomol 4270) 1∶200, Sheep anti-Vangl2 (R&D Systems AF4815) 1∶100, and Rabbit anti-Par-3 (Millipore 07-330) 1∶100.

    Article Title: EPH Receptor Tyrosine Kinases Regulate Epithelial Morphogenesis and Phosphorylate the PAR-3 Scaffold Protein to Modulate Downstream Signaling Networks
    Article Snippet: Primary antibodies used were: rabbit anti-FLAG (Sigma-Aldrich, #F7425), mouse anti-pan phosphotyrosine 4G10 (Millipore, #05-321), mouse anti-GFP (Abcam, #290), mouse anti-EPHA4 (BD Biosciences, #610471), goat anti-EPHB2 (R&D Systems, # AF467), goat anti-EPHA1 (R&D Systems, #AF638), mouse anti-EPHA2 (Millipore, #05-480), goat anti-EPHB4 (R&D Systems, #AF3038), mouse anti-PKCι (BD, #610175), rabbit anti-CSK (Cell Signaling Technology, #4980), rabbit anti-PAR-3 (Millipore, #07-330), mouse anti-actin (Cell Signaling Technology, #3700).

    Article Title: Phosphorylation by Aurora kinase A facilitates cortical-cytoplasmic dynamics of Par-3 in asymmetric division of radial glia progenitors
    Article Snippet: Zebrafish embryos were treated with an AurkA inhibitor (MedChemExpress, MK-5108, HY-13252, CAS no. 1010085-13-8) from 22 to 24 hpf for various durations before imaging (10, 20, 30, 40, 50, 60, and 120 min).

    Article Title: EPH receptor tyrosine kinases phosphorylate the PAR-3 scaffold protein to modulate downstream signaling networks.
    Article Snippet: Primary antibodies used were: rabbit anti-FLAG (Sigma-Aldrich, #F7425), mouse anti-pan phosphotyrosine 4G10 (Sigma-Aldrich, #05–321), mouse anti-GFP (Thermo Fisher Scientific, #A-11122, Roche, #1181446001), mouse anti-EPHA4 (BD Biosciences, #610471), goat anti-EPHB2 (R&D Systems, # AF467), rabbit anti-CSK (Cell Signaling Technology, #4980), rabbit anti-PAR-3 (Millipore, #07–330), rabbit anti-LIMK2 (Cell Signaling Technology, #3845), rabbit anti-phospho-SRC (Tyr527) (Cell Signaling Technology, #2105), mouse anti-SRC (Cell Signaling Technology, #2110) mouse anti-Actin (Cell Signaling Technology, #3700), mouse anti-Tubulin (Cell Signaling Technology, #3873).

    Activity Assay:


    Article Title: Polarized endosome dynamics engage cytosolic Par-3 and dynein during asymmetric division
    Article Snippet: Primary antibodies used in this study were: mouse anti-Dld (Abcam ab73331; RRID:AB_1268496; lot GR115501-3, 1:200 dilution for immunostaining) , chicken anti-GFP (Abcam ab13970; RRID:AB_300798, lot GR3190550-20, 1:500 dilution for immunostaining), rabbit anti-Par-3 (Millipore 07-330; RRID:AB_2101325; lot 3322358, 1:500 for immunostaining)(validated in this study), guinea pig anti-DLIC-Cter (a gift from D. T. Uemura, 1:100 for immunostaining and 1:500 for western blotting, validated in this study) , rabbit anti-α-Tubulin (Santa Cruz SC-12462; RRID:AB_2241125, lot A2907, 1:1000 for western blotting).

    Article Title: Nodal Dependent Differential Localisation of Dishevelled-2 Demarcates Regions of Differing Cell Behaviour in the Visceral Endoderm
    Article Snippet: Primary antibodies used were: Rabbit anti-ZO-1 (Zymed laboratories 61-7300) 1∶100, Rabbit anti-Myosin IIA (Sigma M8064) 1∶100, Rat Anti-Uvomorulin/E-cadherin (Sigma U3254) 1∶200, Rabbit anti-Dishevelled-2 (Biomol 4270) 1∶200, Sheep anti-Vangl2 (R&D Systems AF4815) 1∶100, and Rabbit anti-Par-3 (Millipore 07-330) 1∶100.

    Article Title: EPH Receptor Tyrosine Kinases Regulate Epithelial Morphogenesis and Phosphorylate the PAR-3 Scaffold Protein to Modulate Downstream Signaling Networks
    Article Snippet: Primary antibodies used were: rabbit anti-FLAG (Sigma-Aldrich, #F7425), mouse anti-pan phosphotyrosine 4G10 (Millipore, #05-321), mouse anti-GFP (Abcam, #290), mouse anti-EPHA4 (BD Biosciences, #610471), goat anti-EPHB2 (R&D Systems, # AF467), goat anti-EPHA1 (R&D Systems, #AF638), mouse anti-EPHA2 (Millipore, #05-480), goat anti-EPHB4 (R&D Systems, #AF3038), mouse anti-PKCι (BD, #610175), rabbit anti-CSK (Cell Signaling Technology, #4980), rabbit anti-PAR-3 (Millipore, #07-330), mouse anti-actin (Cell Signaling Technology, #3700).

    Article Title: Phosphorylation by Aurora kinase A facilitates cortical-cytoplasmic dynamics of Par-3 in asymmetric division of radial glia progenitors
    Article Snippet: Zebrafish embryos were treated with an AurkA inhibitor (MedChemExpress, MK-5108, HY-13252, CAS no. 1010085-13-8) from 22 to 24 hpf for various durations before imaging (10, 20, 30, 40, 50, 60, and 120 min).

    Article Title: EPH receptor tyrosine kinases phosphorylate the PAR-3 scaffold protein to modulate downstream signaling networks.
    Article Snippet: Primary antibodies used were: rabbit anti-FLAG (Sigma-Aldrich, #F7425), mouse anti-pan phosphotyrosine 4G10 (Sigma-Aldrich, #05–321), mouse anti-GFP (Thermo Fisher Scientific, #A-11122, Roche, #1181446001), mouse anti-EPHA4 (BD Biosciences, #610471), goat anti-EPHB2 (R&D Systems, # AF467), rabbit anti-CSK (Cell Signaling Technology, #4980), rabbit anti-PAR-3 (Millipore, #07–330), rabbit anti-LIMK2 (Cell Signaling Technology, #3845), rabbit anti-phospho-SRC (Tyr527) (Cell Signaling Technology, #2105), mouse anti-SRC (Cell Signaling Technology, #2110) mouse anti-Actin (Cell Signaling Technology, #3700), mouse anti-Tubulin (Cell Signaling Technology, #3873).

    Labeling:


    Article Title: Polarized endosome dynamics engage cytosolic Par-3 and dynein during asymmetric division
    Article Snippet: Primary antibodies used in this study were: mouse anti-Dld (Abcam ab73331; RRID:AB_1268496; lot GR115501-3, 1:200 dilution for immunostaining) , chicken anti-GFP (Abcam ab13970; RRID:AB_300798, lot GR3190550-20, 1:500 dilution for immunostaining), rabbit anti-Par-3 (Millipore 07-330; RRID:AB_2101325; lot 3322358, 1:500 for immunostaining)(validated in this study), guinea pig anti-DLIC-Cter (a gift from D. T. Uemura, 1:100 for immunostaining and 1:500 for western blotting, validated in this study) , rabbit anti-α-Tubulin (Santa Cruz SC-12462; RRID:AB_2241125, lot A2907, 1:1000 for western blotting).

    Article Title: Nodal Dependent Differential Localisation of Dishevelled-2 Demarcates Regions of Differing Cell Behaviour in the Visceral Endoderm
    Article Snippet: Primary antibodies used were: Rabbit anti-ZO-1 (Zymed laboratories 61-7300) 1∶100, Rabbit anti-Myosin IIA (Sigma M8064) 1∶100, Rat Anti-Uvomorulin/E-cadherin (Sigma U3254) 1∶200, Rabbit anti-Dishevelled-2 (Biomol 4270) 1∶200, Sheep anti-Vangl2 (R&D Systems AF4815) 1∶100, and Rabbit anti-Par-3 (Millipore 07-330) 1∶100.

    Article Title: EPH Receptor Tyrosine Kinases Regulate Epithelial Morphogenesis and Phosphorylate the PAR-3 Scaffold Protein to Modulate Downstream Signaling Networks
    Article Snippet: Primary antibodies used were: rabbit anti-FLAG (Sigma-Aldrich, #F7425), mouse anti-pan phosphotyrosine 4G10 (Millipore, #05-321), mouse anti-GFP (Abcam, #290), mouse anti-EPHA4 (BD Biosciences, #610471), goat anti-EPHB2 (R&D Systems, # AF467), goat anti-EPHA1 (R&D Systems, #AF638), mouse anti-EPHA2 (Millipore, #05-480), goat anti-EPHB4 (R&D Systems, #AF3038), mouse anti-PKCι (BD, #610175), rabbit anti-CSK (Cell Signaling Technology, #4980), rabbit anti-PAR-3 (Millipore, #07-330), mouse anti-actin (Cell Signaling Technology, #3700).

    Article Title: Phosphorylation by Aurora kinase A facilitates cortical-cytoplasmic dynamics of Par-3 in asymmetric division of radial glia progenitors
    Article Snippet: Zebrafish embryos were treated with an AurkA inhibitor (MedChemExpress, MK-5108, HY-13252, CAS no. 1010085-13-8) from 22 to 24 hpf for various durations before imaging (10, 20, 30, 40, 50, 60, and 120 min).

    Article Title: EPH receptor tyrosine kinases phosphorylate the PAR-3 scaffold protein to modulate downstream signaling networks.
    Article Snippet: Primary antibodies used were: rabbit anti-FLAG (Sigma-Aldrich, #F7425), mouse anti-pan phosphotyrosine 4G10 (Sigma-Aldrich, #05–321), mouse anti-GFP (Thermo Fisher Scientific, #A-11122, Roche, #1181446001), mouse anti-EPHA4 (BD Biosciences, #610471), goat anti-EPHB2 (R&D Systems, # AF467), rabbit anti-CSK (Cell Signaling Technology, #4980), rabbit anti-PAR-3 (Millipore, #07–330), rabbit anti-LIMK2 (Cell Signaling Technology, #3845), rabbit anti-phospho-SRC (Tyr527) (Cell Signaling Technology, #2105), mouse anti-SRC (Cell Signaling Technology, #2110) mouse anti-Actin (Cell Signaling Technology, #3700), mouse anti-Tubulin (Cell Signaling Technology, #3873).

    Two Tailed Test:


    Article Title: Polarized endosome dynamics engage cytosolic Par-3 and dynein during asymmetric division
    Article Snippet: Primary antibodies used in this study were: mouse anti-Dld (Abcam ab73331; RRID:AB_1268496; lot GR115501-3, 1:200 dilution for immunostaining) , chicken anti-GFP (Abcam ab13970; RRID:AB_300798, lot GR3190550-20, 1:500 dilution for immunostaining), rabbit anti-Par-3 (Millipore 07-330; RRID:AB_2101325; lot 3322358, 1:500 for immunostaining)(validated in this study), guinea pig anti-DLIC-Cter (a gift from D. T. Uemura, 1:100 for immunostaining and 1:500 for western blotting, validated in this study) , rabbit anti-α-Tubulin (Santa Cruz SC-12462; RRID:AB_2241125, lot A2907, 1:1000 for western blotting).

    Article Title: Nodal Dependent Differential Localisation of Dishevelled-2 Demarcates Regions of Differing Cell Behaviour in the Visceral Endoderm
    Article Snippet: Primary antibodies used were: Rabbit anti-ZO-1 (Zymed laboratories 61-7300) 1∶100, Rabbit anti-Myosin IIA (Sigma M8064) 1∶100, Rat Anti-Uvomorulin/E-cadherin (Sigma U3254) 1∶200, Rabbit anti-Dishevelled-2 (Biomol 4270) 1∶200, Sheep anti-Vangl2 (R&D Systems AF4815) 1∶100, and Rabbit anti-Par-3 (Millipore 07-330) 1∶100.

    Article Title: EPH Receptor Tyrosine Kinases Regulate Epithelial Morphogenesis and Phosphorylate the PAR-3 Scaffold Protein to Modulate Downstream Signaling Networks
    Article Snippet: Primary antibodies used were: rabbit anti-FLAG (Sigma-Aldrich, #F7425), mouse anti-pan phosphotyrosine 4G10 (Millipore, #05-321), mouse anti-GFP (Abcam, #290), mouse anti-EPHA4 (BD Biosciences, #610471), goat anti-EPHB2 (R&D Systems, # AF467), goat anti-EPHA1 (R&D Systems, #AF638), mouse anti-EPHA2 (Millipore, #05-480), goat anti-EPHB4 (R&D Systems, #AF3038), mouse anti-PKCι (BD, #610175), rabbit anti-CSK (Cell Signaling Technology, #4980), rabbit anti-PAR-3 (Millipore, #07-330), mouse anti-actin (Cell Signaling Technology, #3700).

    Article Title: Phosphorylation by Aurora kinase A facilitates cortical-cytoplasmic dynamics of Par-3 in asymmetric division of radial glia progenitors
    Article Snippet: Zebrafish embryos were treated with an AurkA inhibitor (MedChemExpress, MK-5108, HY-13252, CAS no. 1010085-13-8) from 22 to 24 hpf for various durations before imaging (10, 20, 30, 40, 50, 60, and 120 min).

    Article Title: EPH receptor tyrosine kinases phosphorylate the PAR-3 scaffold protein to modulate downstream signaling networks.
    Article Snippet: Primary antibodies used were: rabbit anti-FLAG (Sigma-Aldrich, #F7425), mouse anti-pan phosphotyrosine 4G10 (Sigma-Aldrich, #05–321), mouse anti-GFP (Thermo Fisher Scientific, #A-11122, Roche, #1181446001), mouse anti-EPHA4 (BD Biosciences, #610471), goat anti-EPHB2 (R&D Systems, # AF467), rabbit anti-CSK (Cell Signaling Technology, #4980), rabbit anti-PAR-3 (Millipore, #07–330), rabbit anti-LIMK2 (Cell Signaling Technology, #3845), rabbit anti-phospho-SRC (Tyr527) (Cell Signaling Technology, #2105), mouse anti-SRC (Cell Signaling Technology, #2110) mouse anti-Actin (Cell Signaling Technology, #3700), mouse anti-Tubulin (Cell Signaling Technology, #3873).

    Injection:


    Article Title: Polarized endosome dynamics engage cytosolic Par-3 and dynein during asymmetric division
    Article Snippet: Primary antibodies used in this study were: mouse anti-Dld (Abcam ab73331; RRID:AB_1268496; lot GR115501-3, 1:200 dilution for immunostaining) , chicken anti-GFP (Abcam ab13970; RRID:AB_300798, lot GR3190550-20, 1:500 dilution for immunostaining), rabbit anti-Par-3 (Millipore 07-330; RRID:AB_2101325; lot 3322358, 1:500 for immunostaining)(validated in this study), guinea pig anti-DLIC-Cter (a gift from D. T. Uemura, 1:100 for immunostaining and 1:500 for western blotting, validated in this study) , rabbit anti-α-Tubulin (Santa Cruz SC-12462; RRID:AB_2241125, lot A2907, 1:1000 for western blotting).

    Article Title: Nodal Dependent Differential Localisation of Dishevelled-2 Demarcates Regions of Differing Cell Behaviour in the Visceral Endoderm
    Article Snippet: Primary antibodies used were: Rabbit anti-ZO-1 (Zymed laboratories 61-7300) 1∶100, Rabbit anti-Myosin IIA (Sigma M8064) 1∶100, Rat Anti-Uvomorulin/E-cadherin (Sigma U3254) 1∶200, Rabbit anti-Dishevelled-2 (Biomol 4270) 1∶200, Sheep anti-Vangl2 (R&D Systems AF4815) 1∶100, and Rabbit anti-Par-3 (Millipore 07-330) 1∶100.

    Article Title: EPH Receptor Tyrosine Kinases Regulate Epithelial Morphogenesis and Phosphorylate the PAR-3 Scaffold Protein to Modulate Downstream Signaling Networks
    Article Snippet: Primary antibodies used were: rabbit anti-FLAG (Sigma-Aldrich, #F7425), mouse anti-pan phosphotyrosine 4G10 (Millipore, #05-321), mouse anti-GFP (Abcam, #290), mouse anti-EPHA4 (BD Biosciences, #610471), goat anti-EPHB2 (R&D Systems, # AF467), goat anti-EPHA1 (R&D Systems, #AF638), mouse anti-EPHA2 (Millipore, #05-480), goat anti-EPHB4 (R&D Systems, #AF3038), mouse anti-PKCι (BD, #610175), rabbit anti-CSK (Cell Signaling Technology, #4980), rabbit anti-PAR-3 (Millipore, #07-330), mouse anti-actin (Cell Signaling Technology, #3700).

    Article Title: Phosphorylation by Aurora kinase A facilitates cortical-cytoplasmic dynamics of Par-3 in asymmetric division of radial glia progenitors
    Article Snippet: Zebrafish embryos were treated with an AurkA inhibitor (MedChemExpress, MK-5108, HY-13252, CAS no. 1010085-13-8) from 22 to 24 hpf for various durations before imaging (10, 20, 30, 40, 50, 60, and 120 min).

    Article Title: EPH receptor tyrosine kinases phosphorylate the PAR-3 scaffold protein to modulate downstream signaling networks.
    Article Snippet: Primary antibodies used were: rabbit anti-FLAG (Sigma-Aldrich, #F7425), mouse anti-pan phosphotyrosine 4G10 (Sigma-Aldrich, #05–321), mouse anti-GFP (Thermo Fisher Scientific, #A-11122, Roche, #1181446001), mouse anti-EPHA4 (BD Biosciences, #610471), goat anti-EPHB2 (R&D Systems, # AF467), rabbit anti-CSK (Cell Signaling Technology, #4980), rabbit anti-PAR-3 (Millipore, #07–330), rabbit anti-LIMK2 (Cell Signaling Technology, #3845), rabbit anti-phospho-SRC (Tyr527) (Cell Signaling Technology, #2105), mouse anti-SRC (Cell Signaling Technology, #2110) mouse anti-Actin (Cell Signaling Technology, #3700), mouse anti-Tubulin (Cell Signaling Technology, #3873).

    Microscopy:


    Article Title: Polarized endosome dynamics engage cytosolic Par-3 and dynein during asymmetric division
    Article Snippet: Primary antibodies used in this study were: mouse anti-Dld (Abcam ab73331; RRID:AB_1268496; lot GR115501-3, 1:200 dilution for immunostaining) , chicken anti-GFP (Abcam ab13970; RRID:AB_300798, lot GR3190550-20, 1:500 dilution for immunostaining), rabbit anti-Par-3 (Millipore 07-330; RRID:AB_2101325; lot 3322358, 1:500 for immunostaining)(validated in this study), guinea pig anti-DLIC-Cter (a gift from D. T. Uemura, 1:100 for immunostaining and 1:500 for western blotting, validated in this study) , rabbit anti-α-Tubulin (Santa Cruz SC-12462; RRID:AB_2241125, lot A2907, 1:1000 for western blotting).

    Article Title: Nodal Dependent Differential Localisation of Dishevelled-2 Demarcates Regions of Differing Cell Behaviour in the Visceral Endoderm
    Article Snippet: Primary antibodies used were: Rabbit anti-ZO-1 (Zymed laboratories 61-7300) 1∶100, Rabbit anti-Myosin IIA (Sigma M8064) 1∶100, Rat Anti-Uvomorulin/E-cadherin (Sigma U3254) 1∶200, Rabbit anti-Dishevelled-2 (Biomol 4270) 1∶200, Sheep anti-Vangl2 (R&D Systems AF4815) 1∶100, and Rabbit anti-Par-3 (Millipore 07-330) 1∶100.

    Article Title: EPH Receptor Tyrosine Kinases Regulate Epithelial Morphogenesis and Phosphorylate the PAR-3 Scaffold Protein to Modulate Downstream Signaling Networks
    Article Snippet: Primary antibodies used were: rabbit anti-FLAG (Sigma-Aldrich, #F7425), mouse anti-pan phosphotyrosine 4G10 (Millipore, #05-321), mouse anti-GFP (Abcam, #290), mouse anti-EPHA4 (BD Biosciences, #610471), goat anti-EPHB2 (R&D Systems, # AF467), goat anti-EPHA1 (R&D Systems, #AF638), mouse anti-EPHA2 (Millipore, #05-480), goat anti-EPHB4 (R&D Systems, #AF3038), mouse anti-PKCι (BD, #610175), rabbit anti-CSK (Cell Signaling Technology, #4980), rabbit anti-PAR-3 (Millipore, #07-330), mouse anti-actin (Cell Signaling Technology, #3700).

    Article Title: Phosphorylation by Aurora kinase A facilitates cortical-cytoplasmic dynamics of Par-3 in asymmetric division of radial glia progenitors
    Article Snippet: Zebrafish embryos were treated with an AurkA inhibitor (MedChemExpress, MK-5108, HY-13252, CAS no. 1010085-13-8) from 22 to 24 hpf for various durations before imaging (10, 20, 30, 40, 50, 60, and 120 min).

    Article Title: EPH receptor tyrosine kinases phosphorylate the PAR-3 scaffold protein to modulate downstream signaling networks.
    Article Snippet: Primary antibodies used were: rabbit anti-FLAG (Sigma-Aldrich, #F7425), mouse anti-pan phosphotyrosine 4G10 (Sigma-Aldrich, #05–321), mouse anti-GFP (Thermo Fisher Scientific, #A-11122, Roche, #1181446001), mouse anti-EPHA4 (BD Biosciences, #610471), goat anti-EPHB2 (R&D Systems, # AF467), rabbit anti-CSK (Cell Signaling Technology, #4980), rabbit anti-PAR-3 (Millipore, #07–330), rabbit anti-LIMK2 (Cell Signaling Technology, #3845), rabbit anti-phospho-SRC (Tyr527) (Cell Signaling Technology, #2105), mouse anti-SRC (Cell Signaling Technology, #2110) mouse anti-Actin (Cell Signaling Technology, #3700), mouse anti-Tubulin (Cell Signaling Technology, #3873).

    In Vivo:


    Article Title: Polarized endosome dynamics engage cytosolic Par-3 and dynein during asymmetric division
    Article Snippet: Primary antibodies used in this study were: mouse anti-Dld (Abcam ab73331; RRID:AB_1268496; lot GR115501-3, 1:200 dilution for immunostaining) , chicken anti-GFP (Abcam ab13970; RRID:AB_300798, lot GR3190550-20, 1:500 dilution for immunostaining), rabbit anti-Par-3 (Millipore 07-330; RRID:AB_2101325; lot 3322358, 1:500 for immunostaining)(validated in this study), guinea pig anti-DLIC-Cter (a gift from D. T. Uemura, 1:100 for immunostaining and 1:500 for western blotting, validated in this study) , rabbit anti-α-Tubulin (Santa Cruz SC-12462; RRID:AB_2241125, lot A2907, 1:1000 for western blotting).

    Article Title: Nodal Dependent Differential Localisation of Dishevelled-2 Demarcates Regions of Differing Cell Behaviour in the Visceral Endoderm
    Article Snippet: Primary antibodies used were: Rabbit anti-ZO-1 (Zymed laboratories 61-7300) 1∶100, Rabbit anti-Myosin IIA (Sigma M8064) 1∶100, Rat Anti-Uvomorulin/E-cadherin (Sigma U3254) 1∶200, Rabbit anti-Dishevelled-2 (Biomol 4270) 1∶200, Sheep anti-Vangl2 (R&D Systems AF4815) 1∶100, and Rabbit anti-Par-3 (Millipore 07-330) 1∶100.

    Article Title: EPH Receptor Tyrosine Kinases Regulate Epithelial Morphogenesis and Phosphorylate the PAR-3 Scaffold Protein to Modulate Downstream Signaling Networks
    Article Snippet: Primary antibodies used were: rabbit anti-FLAG (Sigma-Aldrich, #F7425), mouse anti-pan phosphotyrosine 4G10 (Millipore, #05-321), mouse anti-GFP (Abcam, #290), mouse anti-EPHA4 (BD Biosciences, #610471), goat anti-EPHB2 (R&D Systems, # AF467), goat anti-EPHA1 (R&D Systems, #AF638), mouse anti-EPHA2 (Millipore, #05-480), goat anti-EPHB4 (R&D Systems, #AF3038), mouse anti-PKCι (BD, #610175), rabbit anti-CSK (Cell Signaling Technology, #4980), rabbit anti-PAR-3 (Millipore, #07-330), mouse anti-actin (Cell Signaling Technology, #3700).

    Article Title: Phosphorylation by Aurora kinase A facilitates cortical-cytoplasmic dynamics of Par-3 in asymmetric division of radial glia progenitors
    Article Snippet: Zebrafish embryos were treated with an AurkA inhibitor (MedChemExpress, MK-5108, HY-13252, CAS no. 1010085-13-8) from 22 to 24 hpf for various durations before imaging (10, 20, 30, 40, 50, 60, and 120 min).

    Article Title: EPH receptor tyrosine kinases phosphorylate the PAR-3 scaffold protein to modulate downstream signaling networks.
    Article Snippet: Primary antibodies used were: rabbit anti-FLAG (Sigma-Aldrich, #F7425), mouse anti-pan phosphotyrosine 4G10 (Sigma-Aldrich, #05–321), mouse anti-GFP (Thermo Fisher Scientific, #A-11122, Roche, #1181446001), mouse anti-EPHA4 (BD Biosciences, #610471), goat anti-EPHB2 (R&D Systems, # AF467), rabbit anti-CSK (Cell Signaling Technology, #4980), rabbit anti-PAR-3 (Millipore, #07–330), rabbit anti-LIMK2 (Cell Signaling Technology, #3845), rabbit anti-phospho-SRC (Tyr527) (Cell Signaling Technology, #2105), mouse anti-SRC (Cell Signaling Technology, #2110) mouse anti-Actin (Cell Signaling Technology, #3700), mouse anti-Tubulin (Cell Signaling Technology, #3873).

    Fluorescence:


    Article Title: Polarized endosome dynamics engage cytosolic Par-3 and dynein during asymmetric division
    Article Snippet: Primary antibodies used in this study were: mouse anti-Dld (Abcam ab73331; RRID:AB_1268496; lot GR115501-3, 1:200 dilution for immunostaining) , chicken anti-GFP (Abcam ab13970; RRID:AB_300798, lot GR3190550-20, 1:500 dilution for immunostaining), rabbit anti-Par-3 (Millipore 07-330; RRID:AB_2101325; lot 3322358, 1:500 for immunostaining)(validated in this study), guinea pig anti-DLIC-Cter (a gift from D. T. Uemura, 1:100 for immunostaining and 1:500 for western blotting, validated in this study) , rabbit anti-α-Tubulin (Santa Cruz SC-12462; RRID:AB_2241125, lot A2907, 1:1000 for western blotting).

    Article Title: Nodal Dependent Differential Localisation of Dishevelled-2 Demarcates Regions of Differing Cell Behaviour in the Visceral Endoderm
    Article Snippet: Primary antibodies used were: Rabbit anti-ZO-1 (Zymed laboratories 61-7300) 1∶100, Rabbit anti-Myosin IIA (Sigma M8064) 1∶100, Rat Anti-Uvomorulin/E-cadherin (Sigma U3254) 1∶200, Rabbit anti-Dishevelled-2 (Biomol 4270) 1∶200, Sheep anti-Vangl2 (R&D Systems AF4815) 1∶100, and Rabbit anti-Par-3 (Millipore 07-330) 1∶100.

    Article Title: EPH Receptor Tyrosine Kinases Regulate Epithelial Morphogenesis and Phosphorylate the PAR-3 Scaffold Protein to Modulate Downstream Signaling Networks
    Article Snippet: Primary antibodies used were: rabbit anti-FLAG (Sigma-Aldrich, #F7425), mouse anti-pan phosphotyrosine 4G10 (Millipore, #05-321), mouse anti-GFP (Abcam, #290), mouse anti-EPHA4 (BD Biosciences, #610471), goat anti-EPHB2 (R&D Systems, # AF467), goat anti-EPHA1 (R&D Systems, #AF638), mouse anti-EPHA2 (Millipore, #05-480), goat anti-EPHB4 (R&D Systems, #AF3038), mouse anti-PKCι (BD, #610175), rabbit anti-CSK (Cell Signaling Technology, #4980), rabbit anti-PAR-3 (Millipore, #07-330), mouse anti-actin (Cell Signaling Technology, #3700).

    Article Title: Phosphorylation by Aurora kinase A facilitates cortical-cytoplasmic dynamics of Par-3 in asymmetric division of radial glia progenitors
    Article Snippet: Zebrafish embryos were treated with an AurkA inhibitor (MedChemExpress, MK-5108, HY-13252, CAS no. 1010085-13-8) from 22 to 24 hpf for various durations before imaging (10, 20, 30, 40, 50, 60, and 120 min).

    Article Title: EPH receptor tyrosine kinases phosphorylate the PAR-3 scaffold protein to modulate downstream signaling networks.
    Article Snippet: Primary antibodies used were: rabbit anti-FLAG (Sigma-Aldrich, #F7425), mouse anti-pan phosphotyrosine 4G10 (Sigma-Aldrich, #05–321), mouse anti-GFP (Thermo Fisher Scientific, #A-11122, Roche, #1181446001), mouse anti-EPHA4 (BD Biosciences, #610471), goat anti-EPHB2 (R&D Systems, # AF467), rabbit anti-CSK (Cell Signaling Technology, #4980), rabbit anti-PAR-3 (Millipore, #07–330), rabbit anti-LIMK2 (Cell Signaling Technology, #3845), rabbit anti-phospho-SRC (Tyr527) (Cell Signaling Technology, #2105), mouse anti-SRC (Cell Signaling Technology, #2110) mouse anti-Actin (Cell Signaling Technology, #3700), mouse anti-Tubulin (Cell Signaling Technology, #3873).



    Similar Products

    90
    Millipore rabbit anti-par-3
    Rabbit Anti Par 3, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti-par-3/par3+antibody/pmc12077515-214-31-32
    Average 90 stars, based on 1 article reviews
    rabbit anti-par-3 - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    90
    Millipore anti-par-3 rabbit pab
    (A) Distribution of afadin (green), α-catenin (red), and F-actin (blue) in WT and afadin-KO cells; apical views of WT or afadin-KO MDCK cells cultured for 24 h and stained for the indicated molecules. Dashed lines indicate the position of vertical section. Arrows indicate scanned regions in the densitometric traces. A representative image is shown of six independent experiments. Scale bars, 10 μm. (X-Z) Vertical sections of the upper images. (Bottom, right) Densitometric traces were performed along the arrows. A.U., arbitrary unit. (B) Distribution of α-catenin (green) and β-catenin (magenta) in WT and afadin-KO cells. Merged images are shown; apical views of WT or afadin KO cells cultured for 24 h and stained for α- and β-catenin. Arrowheads indicate the apical cell junctions. Arrows point to the basal edge of the junctions. Scale bars, 10 μm. (X-Z) Vertical sections of the upper images. Scale bars, 10 μm. (C) Quantifications of cell height and tilting extent of LCs. Boxes represent mean ± standard deviation (S.D.), and whiskers indicate maximum and minimum values. Tilting extent; n = 30 for WT; n = 30 for afadin KO. Cell height; n = 95 for WT; n = 102 for afadin KO. Mann-Whitney U -test; **, P ≤ 0.01, ***, P ≤ 0.001. See also figure S2B. (D) Distribution of afadin (green), <t>Par-3</t> (red), and ZO-1 (blue) in WT and afadin-KO cells. WT and afadin KO cells were cultured for 24 h and triple stained with the indicated molecules. Scale bars, 10 μm.
    Anti Par 3 Rabbit Pab, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti-par-3/par3+antibody/bio_rxiv__2024__04__26__591237-237-43-47
    Average 90 stars, based on 1 article reviews
    anti-par-3 rabbit pab - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    96
    Cell Signaling Technology Inc mouse anti par polymer
    KEY RESOURCES TABLE
    Mouse Anti Par Polymer, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti-par-3/Phospho-IRF-3+(Ser396)+Rabbit+mAb/pmc09136538-13-0-4
    Average 96 stars, based on 1 article reviews
    mouse anti par polymer - by Bioz Stars, 2026-10
    96/100 stars
      Buy from Supplier

    90
    Millipore rabbit anti-par-3 (1:500, millipore)
    KEY RESOURCES TABLE
    Rabbit Anti Par 3 (1:500, Millipore), supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti-par-3/par3+antibody/bio_rxiv__2022__03__01__482468-249-71-74
    Average 90 stars, based on 1 article reviews
    rabbit anti-par-3 (1:500, millipore) - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    90
    Millipore anti-rabbit par-3
    KEY RESOURCES TABLE
    Anti Rabbit Par 3, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti-par-3/par3+antibody/pmc08511111-154-58-61
    Average 90 stars, based on 1 article reviews
    anti-rabbit par-3 - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    96
    Santa Cruz Biotechnology rabbit polyclonal anti par 1 h 111
    KEY RESOURCES TABLE
    Rabbit Polyclonal Anti Par 1 H 111, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti-par-3/caspase-3+Antibody/pmc08509732-205-20-25
    Average 96 stars, based on 1 article reviews
    rabbit polyclonal anti par 1 h 111 - by Bioz Stars, 2026-10
    96/100 stars
      Buy from Supplier

    90
    Millipore rabbit anti–par-3
    KEY RESOURCES TABLE
    Rabbit Anti–Par 3, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti-par-3/par3+antibody/pmc08195473-219-38-39
    Average 90 stars, based on 1 article reviews
    rabbit anti–par-3 - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    Image Search Results


    (A) Distribution of afadin (green), α-catenin (red), and F-actin (blue) in WT and afadin-KO cells; apical views of WT or afadin-KO MDCK cells cultured for 24 h and stained for the indicated molecules. Dashed lines indicate the position of vertical section. Arrows indicate scanned regions in the densitometric traces. A representative image is shown of six independent experiments. Scale bars, 10 μm. (X-Z) Vertical sections of the upper images. (Bottom, right) Densitometric traces were performed along the arrows. A.U., arbitrary unit. (B) Distribution of α-catenin (green) and β-catenin (magenta) in WT and afadin-KO cells. Merged images are shown; apical views of WT or afadin KO cells cultured for 24 h and stained for α- and β-catenin. Arrowheads indicate the apical cell junctions. Arrows point to the basal edge of the junctions. Scale bars, 10 μm. (X-Z) Vertical sections of the upper images. Scale bars, 10 μm. (C) Quantifications of cell height and tilting extent of LCs. Boxes represent mean ± standard deviation (S.D.), and whiskers indicate maximum and minimum values. Tilting extent; n = 30 for WT; n = 30 for afadin KO. Cell height; n = 95 for WT; n = 102 for afadin KO. Mann-Whitney U -test; **, P ≤ 0.01, ***, P ≤ 0.001. See also figure S2B. (D) Distribution of afadin (green), Par-3 (red), and ZO-1 (blue) in WT and afadin-KO cells. WT and afadin KO cells were cultured for 24 h and triple stained with the indicated molecules. Scale bars, 10 μm.

    Journal: bioRxiv

    Article Title: Multivalent Afadin Interaction Promotes IDR-Mediated Condensate Formation and Junctional Separation of Epithelial Cells

    doi: 10.1101/2024.04.26.591237

    Figure Lengend Snippet: (A) Distribution of afadin (green), α-catenin (red), and F-actin (blue) in WT and afadin-KO cells; apical views of WT or afadin-KO MDCK cells cultured for 24 h and stained for the indicated molecules. Dashed lines indicate the position of vertical section. Arrows indicate scanned regions in the densitometric traces. A representative image is shown of six independent experiments. Scale bars, 10 μm. (X-Z) Vertical sections of the upper images. (Bottom, right) Densitometric traces were performed along the arrows. A.U., arbitrary unit. (B) Distribution of α-catenin (green) and β-catenin (magenta) in WT and afadin-KO cells. Merged images are shown; apical views of WT or afadin KO cells cultured for 24 h and stained for α- and β-catenin. Arrowheads indicate the apical cell junctions. Arrows point to the basal edge of the junctions. Scale bars, 10 μm. (X-Z) Vertical sections of the upper images. Scale bars, 10 μm. (C) Quantifications of cell height and tilting extent of LCs. Boxes represent mean ± standard deviation (S.D.), and whiskers indicate maximum and minimum values. Tilting extent; n = 30 for WT; n = 30 for afadin KO. Cell height; n = 95 for WT; n = 102 for afadin KO. Mann-Whitney U -test; **, P ≤ 0.01, ***, P ≤ 0.001. See also figure S2B. (D) Distribution of afadin (green), Par-3 (red), and ZO-1 (blue) in WT and afadin-KO cells. WT and afadin KO cells were cultured for 24 h and triple stained with the indicated molecules. Scale bars, 10 μm.

    Article Snippet: The following primary antibodies (Ab) were used: anti-afadin mouse monoclonal Ab (mAb) (#AM26175PU-N, clone 3, OriGene; 1:50 dilution for immunofluorescence, 1:3000 dilution for western blotting 55 ), anti-α-catenin rabbit polyclonal Ab (pAb) (#C2081, Sigma-Aldrich; 1:400 dilution for immunofluorescence, 1:1000 dilution for western blotting), anti-Par-3 rabbit pAb (#07-330, Sigma-Aldrich; 1:100 dilution for immunofluorescence), anti-β-catenin mouse mAb (#MAB2081, clone 5H10 , Sigma-Aldrich; 1:1000 dilution for immunofluorescence), anti-β-catenin rabbit pAb (#C2206, Sigma-Aldrich; 1:800 for immunofluorescence), anti-ZO-1 rat mAb (#sc-33725, clone R40.76 , Santa Cruz Biotechnology; 1:50 dilution for immunofluorescence, 1:1000 dilution for western blotting), anti-HA rat mAb (#11867431001, clone 3F10, Roche; 1:4000 dilution for western blotting), anti-α-tubulin mouse mAb (#T6199, clone DM1A , Sigma-Aldrich, 1:5000 dilution for western blotting), anti-GFP rat mAb (#04404-84, clone GF090R, NacalaiTesque; 2.5 μg/reaction for co-immunoprecipitation).

    Techniques: Cell Culture, Staining, Standard Deviation, MANN-WHITNEY

    KEY RESOURCES TABLE

    Journal: Cell reports

    Article Title: The lactate-NAD + axis activates cancer-associated fibroblasts by downregulating p62

    doi: 10.1016/j.celrep.2022.110792

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: Mouse anti-PAR Polymer , Cell Signaling Technology , Cat# 4947; RRID: AB_823547.

    Techniques: Polymer, Virus, Recombinant, Transfection, Western Blot, Infection, Electroporation, Quantitation Assay, Control, Staining, Real-time Polymerase Chain Reaction, Plasmid Preparation, Software, Imaging, Spectrophotometry, Microscopy